Functional analysis of CHX21: a putative sodium transporter in Arabidopsis

Journal of Experimental Botany
D HallJ Pritchard

Abstract

The functional role of CHX21, a member of the Arabidopsis thaliana CHX cation transporter family, has been investigated in plants growing under "ideal" conditions and in the presence of elevated NaCl levels. In public databases, AtCHX21 (At2g31910) is annotated as a putative Na+/H+ antiporter. In this study, Southern analysis was used to identify a genotype that contained a single transposon insertion within its genome; using PCR, this insertion was shown to be within the CHX21 locus. No CHX21 transcript was detectable in Atchx21 (mutant) plants using RT-PCR. In the absence of salt stress, Atchx21 showed significant quantitative differences from the wild type (AtCHX21) in development with respect to characters such as rosette width and flowering time. In the presence of 50 mM NaCl, (i) roots of Atchx21 elongated more slowly than the wild type, (ii) the leaf sap Na+ concentration was significantly lower in Atchx21 compared with the wild type, and (iii) the concentra) in the xylem was lower compared with the wild type. The concentration of Na+ exported from the leaf in the phloem was unchanged. Thus, loading of Na+ into the root xylem could explain changes in leaf concentration of Na+. This hypothesis was supported by immunolocal...Continue Reading

References

Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·R A GaxiolaG R Fink
Mar 15, 2001·Biochimica Et Biophysica Acta·E PadanN Dover
Aug 2, 2001·Nature Biotechnology·H X Zhang, E Blumwald
Aug 14, 2001·Plant Physiology·P MäserM L Guerinot
Sep 5, 2001·The Plant Journal : for Cell and Molecular Biology·V VitartJ F Harper
Apr 16, 2003·The Journal of Biological Chemistry·Kees VenemaJuan Pedro Donaire
Oct 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Toshio YamaguchiEduardo Blumwald
Jun 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chun-Peng SongJian-Kang Zhu
Sep 3, 2004·The Plant Journal : for Cell and Molecular Biology·Françoise CellierFrancine Casse
May 1, 1994·The New Phytologist·Jeremy Pritchard

❮ Previous
Next ❯

Citations

Oct 7, 2011·Journal of Experimental Botany·A R EvansH J Newbury
Mar 6, 2007·Plant Physiology·Senthilkumar PadmanabanHeven Sze
Aug 5, 2008·Plant Physiology·Jian ZhaoKendal D Hirschi
Mar 19, 2014·Trends in Plant Science·Ulrich DeinleinJulian I Schroeder
Oct 24, 2013·Journal of Experimental Botany·Frans J M Maathuis
Jan 1, 2012·ISRN Molecular Biology·Faïçal Brini, Khaled Masmoudi
Apr 27, 2007·FEBS Letters·Maris P Apse, Eduardo Blumwald
Jun 21, 2008·The New Phytologist·Timothy J Flowers, Timothy D Colmer
Aug 30, 2008·Physiologia Plantarum·Sergey Shabala, Tracey A Cuin
Dec 18, 2009·The Plant Journal : for Cell and Molecular Biology·Sergey ShabalaLars H Wegner
Jan 8, 2014·Journal of Integrative Plant Biology·Eri Adams, Ryoung Shin
Nov 10, 2009·Plant, Cell & Environment·Natasha L Teakle, Stephen D Tyerman
Dec 9, 2009·Plant, Cell & Environment·Darren Craig Plett, Inge Skrumsager Møller
May 4, 2012·Biochimica Et Biophysica Acta·María Nieves Aranda-SiciliaKees Venema
Oct 4, 2014·Frontiers in Plant Science·Frans J M MaathuisJuan Patishtan
Mar 3, 2018·International Journal of Molecular Sciences·Monika KeishamSatish C Bhatla
Jul 10, 2014·Nature Communications·Xinpeng QiHon-Ming Lam
Nov 7, 2019·Plant & Cell Physiology·Mahvash ZareiLana Shabala
Mar 14, 2020·Annual Review of Plant Biology·Eva van ZelmChrista Testerink
Aug 10, 2020·Physiologia Plantarum·Insha AminParvaiz Ahmad
Jan 18, 2019·Plants·Emilie AubryRozenn Le Hir
Oct 31, 2020·Frontiers in Plant Science·Stanislav V IsayenkovSergey Shabala
Feb 2, 2021·Plant Physiology and Biochemistry : PPB·Sadam HussainNaeem Ahmad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.