Functional analysis of genomic DNA, cDNA, and nucleotide sequence of the mature C-type natriuretic peptide gene in vascular cells

Arteriosclerosis, Thrombosis, and Vascular Biology
J PelisekS Nikol

Abstract

The aim of this study was to investigate the effect of various C-type natriuretic peptide (CNP) sequences (genomic DNA [CNPDNA], cDNA derived from mRNA [CNPcDNA], and sequence coding for 22 amino acids of the mature CNP [CNP22aa]) on the growth of primary porcine vascular cells. Gene transfer was performed with cationic lipid DOCSPER or linear polyethylenimine. All 3 CNP sequences led to significant inhibition of smooth muscle cell (SMC) proliferation. In contrast, significant stimulation of cell growth was observed in endothelial cells (ECs) using CNPDNA or CNPcDNA but not CNP22aa. In a porcine restenosis model, a significant reduction in neointima hyperplasia was found 3 months after application of the CNPcDNA vector compared with the control transfection. The results demonstrate that the first intron in the CNP sequence does not contain any additional enhancer-binding sites. However, the signal sequence is indispensable for secretion of CNP and its appropriate physiological function. Furthermore, the results show for the first time the therapeutic effect of CNP using liposome-mediated gene transfer and local adventitial delivery. Advantages of the CNP gene are its dual effects with inhibition of SMC proliferation and simulta...Continue Reading

References

Jan 1, 1996·Peptides·C S BarrA D Struthers
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·W T GodbeyA G Mikos
Mar 15, 2000·The International Journal of Biochemistry & Cell Biology·M Okada-BanJ Jouanneau
Apr 12, 2000·The International Journal of Angiology : Official Publication of the International College of Angiology, Inc·S NikolS Armeanu
Jun 6, 2000·Advanced Drug Delivery Reviews·M B BallyD L Reimer
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·S ArmeanuS Nikol
Apr 18, 2001·Cellular Signalling·M Silberbach, C T Roberts
Jul 31, 2001·Cardiovascular Research·B C Kone
Jul 31, 2001·Cardiovascular Research·T SuzukiY Yazaki
Dec 18, 2001·Advanced Drug Delivery Reviews·R KircheisE Wagner
Feb 28, 2002·Endocrinology·Alexandra K KiemerAngelika M Vollmar
Oct 29, 2002·Bioelectrochemistry·R I ZhdanovV V Vlassov
Nov 19, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jaroslav PelisekSigrid Nikol

❮ Previous
Next ❯

Citations

Apr 4, 2013·Journal of Negative Results in Biomedicine·Andreas KuehnlHans-Henning Eckstein
Oct 18, 2006·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·Wen TianSigrid Nikol
Jun 24, 2008·Endothelium : Journal of Endothelial Cell Research·Alexandra Theresia FuchsSigrid Nikol
Jun 16, 2012·International Wound Journal·Andreas KuehnlStefan Langer
Apr 13, 2005·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·Andreas KühnlSigrid Nikol

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.