Functional analysis of I alpha promoter regions of multiple IgA heavy chain genes

The Journal of Immunology : Official Journal of the American Association of Immunologists
H Spieker-PoletKatherine L Knight

Abstract

The 13 nonallelic IgA H chain genes of rabbit are differentially expressed in vivo. They can be grouped into those expressed at high levels (Calpha4, Calpha5, Calpha6, Calpha9, Calpha10, Calpha12, and Calpha13), those expressed at low levels (Calpha1, Calpha2, Calpha7, and Calpha11), and those that are not expressed (Calpha3 and Calpha8). We tested whether the differential in vivo expression is due to differential responses of the Ialpha promoters to TGF-beta stimulation. We stimulated the rabbit B cell line 55D1 with TGF-beta and, using single-cell RT-PCR, found that expression of germline (GL) transcripts of alpha3 and alpha8 could not be induced. By luciferase reporter gene assay and EMSA we found that the promoters of the unexpressed isotypes Calpha3 and Calpha8 are defective, thereby explaining the absence of IgA3 and IgA8 in vivo. When comparing the promoter activities of the other isotypes we found that the activities did not reflect the degree of in vivo expression. Instead, the promoters of the isotypes expressed at high or low levels promoted expression of the luciferase gene to a similar degree, except for the Ialpha4 promoter, which had much higher activity. Also the degree to which TGF-beta induced GL expression of...Continue Reading

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D A LebmanF D Lee
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R D SchneidermanK L Knight
Jan 1, 1993·Advances in Immunology·R L CoffmanP Rothman
Jan 1, 1996·Advances in Immunology·J Stavnezer
Mar 1, 1997·Immunity·C M SnapperP Zelazowski
Mar 4, 1999·European Journal of Immunology·X Q XieT Grundström
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y HuL Hammarström
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·V V VolginaK L Knight

❮ Previous
Next ❯

Citations

Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul J JasperKatherine L Knight
Jul 8, 2005·International Immunology·Veronica VolginaKatherine L Knight
Nov 4, 2004·Seminars in Immunology·Janet Stavnezer, Chris T Amemiya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
H Spieker-PoletK L Knight
The Journal of Immunology : Official Journal of the American Association of Immunologists
Veronica VolginaK L Knight
International Immunology
Veronica VolginaKatherine L Knight
The Journal of Immunology : Official Journal of the American Association of Immunologists
P J Gearhart, J J Cebra
© 2022 Meta ULC. All rights reserved