Functional Analysis of Ion Transport Properties and Salt Tolerance Mechanisms of RtHKT1 from the Recretohalophyte Reaumuria trigyna

Plant & Cell Physiology
Ningning LiYingchun Wang

Abstract

Reaumuria trigyna is an endangered recretohalophyte and a small archaic feral shrub that is endemic to arid and semi-arid plateau regions of Inner Mongolia, China. Based on transcriptomic data, we isolated a high-affinity potassium transporter gene (RtHKT1) from R. trigyna, which encoded a plasma membrane-localized protein. RtHKT1 was rapidly up-regulated by high Na+ or low K+ and exhibited different tissue-specific expression patterns before and after stress treatment. Transgenic yeast showed tolerance to high Na+ or low K+, while transgenic Arabidopsis exhibited tolerance to high Na+ and sensitivity to high K+, or high Na+-low K+, confirming that Na+ tolerance in transgenic Arabidopsis depends on a sufficient external K+ concentration. Under external high Na+, high K+ and low K+ conditions, transgenic yeast accumulated more Na+-K+, Na+ and K+, while transgenic Arabidopsis accumulated less Na+-more K+, more Na+ and more Na+-K+, respectively, indicating that the ion transport properties of RtHKT1 depend on the external Na+-K+ environment. Salt stress induced up-regulation of some ion transporter genes (AtSOS1/AtHAK5/AtKUP5-6), as well as down-regulation of some genes (AtNHX1/AtAVP1/AtKUP9-12), revealing that multi-ion-transport...Continue Reading

References

Nov 1, 1996·The Plant Journal : for Cell and Molecular Biology·W GassmanJ I Schroeder
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G T KimH Uchimiya
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·H ShiJ K Zhu
Jun 30, 2000·Current Opinion in Cell Biology·E Blumwald
Feb 15, 2001·Trends in Plant Science·J K Zhu
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Francisco J QuinteroJose M Pardo
Nov 29, 2002·Journal of Cell Science·Vadim DemidchikJulia M Davies
Jun 11, 2003·The Plant Journal : for Cell and Molecular Biology·Blanca GarciadeblásAlonso Rodríguez-Navarro
Oct 16, 2003·Plant Molecular Biology·Hua SuHans J Bohnert
Sep 7, 2004·Plant Physiology·Ana RusPaul M Hasegawa
Oct 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ji-Ming GongJulian I Schroeder
Sep 13, 2005·Nature Genetics·Zhong-Hai RenHong-Xuan Lin
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology· SunarpiNobuyuki Uozumi
Jul 1, 2006·Trends in Plant Science·J Damien PlattenMark Tester
Feb 27, 2007·Plant Physiology·Caitlin S ByrtRana Munns
Feb 28, 2007·Plant, Cell & Environment·Romola Jane DavenportMark Tester
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Brook K NelsonAndreas Nebenführ
Mar 19, 2008·Physiologia Plantarum·Gad MillerRon Mittler
Jun 21, 2008·The New Phytologist·Timothy J Flowers, Timothy D Colmer
Feb 14, 2009·Journal of Experimental Botany·Matthias Josef AffenzellerUrsula Lütz-Meindl
Mar 10, 2009·Journal of Experimental Botany·Sergey Shabala
Jun 2, 2009·Plant Physiology·Mehdi JabnouneAnne-Aliénor Véry
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Dec 2, 2010·The New Phytologist·Herbert J Kronzucker, Dev T Britto
Mar 13, 2012·Nature Biotechnology·Rana MunnsMatthew Gilliham
Jul 10, 2012·Plant Physiology·Ali SassiAnne-Aliénor Véry
Oct 4, 2012·Plant Physiology and Biochemistry : PPB·Ana María Velarde-BuendíaIgor Pottosin
Feb 12, 2013·The Plant Cell·Yuriko OsakabeKazuko Yamaguchi-Shinozaki

❮ Previous
Next ❯

Citations

Dec 24, 2019·Plant Signaling & Behavior·Cunxian Fan
Apr 23, 2020·Physiologia Plantarum·Sahana BasuGautam Kumar
Aug 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ge LiZongyun Li
Aug 4, 2020·Plant, Cell & Environment·Lukasz KotulaTimothy J Flowers
Jul 29, 2021·Plant Science : an International Journal of Experimental Plant Biology·Binjie MaYingchun Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.