Functional analysis of late-budding domain activity associated with the PSAP motif within the vesicular stomatitis virus M protein

Journal of Virology
Takashi IrieRonald N Harty

Abstract

A PPPY motif within the M protein of vesicular stomatitis virus (VSV) functions as a late-budding domain (L-domain); however, L-domain activity has yet to be associated with a downstream PSAP motif. VSV recombinants with mutations in the PPPY and/or PSAP motif were recovered by reverse genetics and examined for growth kinetics, plaque size, and budding efficiency by electron microscopy. Results indicate that unlike the PPPY motif, the PSAP motif alone does not possess L-domain activity. Finally, the insertion of the human immunodeficiency virus type 1 p6 L-domain and flanking sequences into the PSAP region of M protein rescued budding of a PPPY mutant of VSV to wild-type levels.

References

Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P WhelanG T Wertz
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N HartyF P Hayes
Apr 21, 2001·Virology·J TimminsW Weissenhorn
May 3, 2001·Journal of Virology·L D JasenoskyY Kawaoka
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L VerPlankC A Carter
Oct 17, 2001·Journal of Virology·R N HartyM J Schnell
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dimiter G DemirovEric O Freed
Apr 23, 2002·Journal of Virology·Eric O Freed
Apr 23, 2002·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
Apr 26, 2002·Trends in Microbiology·Carol A Carter
May 15, 2002·The EMBO Journal·Owen PornillosWesley I Sundquist
Oct 29, 2003·Journal of Virology·Juan Martin-Serrano, Paul D Bieniasz

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Citations

Apr 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·René AssenbergJonathan M Grimes
Mar 7, 2008·Journal of Virology·Alireza GholamiHervé Bourhy
Jul 30, 2005·Journal of Virology·Gabriele NeumannYoshihiro Kawaoka
Jan 1, 2012·Uirusu·Akira Nishizono, Kentaro Yamada
Dec 4, 2012·Viruses·Shuzo Urata, Jiro Yasuda
Jul 27, 2010·Critical Reviews in Biochemistry and Molecular Biology·James H Hurley
Jan 29, 2016·Reviews in Medical Virology·Laura de Armas-RilloAgustín Valenzuela-Fernández
Jul 14, 2010·Virus Research·Rie Watanabe, Robert A Lamb
Dec 23, 2011·The Journal of General Virology·Linda ObiangYves Gaudin
Jul 21, 2015·Microbiology and Immunology·Chisato Narahara, Jiro Yasuda
Nov 13, 2008·Virology Journal·Jared R PatchChristopher C Broder
Dec 15, 2010·Cell·James H HurleyBartosz Różycki
Dec 8, 2007·Virology·Benjamin J Chen, Robert A Lamb
May 17, 2005·Virology·Takashi IrieRonald N Harty
Aug 12, 2014·PloS One·Megan Moerdyk-SchauweckerValery Z Grdzelishvili
Dec 30, 2008·PLoS Pathogens·Stephen C GrahamHervé Bourhy
Jul 6, 2016·Bioorganic & Medicinal Chemistry Letters·H Marie LoughranAllen B Reitz
Nov 30, 2004·Virus Research·Dimiter G Demirov, Eric O Freed

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