Functional analysis of lymphostatin homologues in enterohaemorrhagic Escherichia coli.

FEMS Microbiology Letters
Abu-Bakr Abu-MedianMark P Stevens

Abstract

Enteropathogenic Escherichia coli contain a large chromosomal gene (lifA) that encodes lymphostatin, a predicted 365 kDa protein that inhibits the mitogen-activated proliferation of peripheral blood lymphocytes and lamina propria mononuclear cells and the synthesis of proinflammatory cytokines. Non-O157 serotypes of enterohaemorrhagic E. coli (EHEC) contain a highly homologous gene, designated efa1 (EHEC factor for adherence), which influences adherence to epithelial cells in vitro and intestinal colonization in calves. Serotype O157:H7 EHEC strains contain a truncated version of this gene (efa1') and a pO157-encoded homologue of lifA/efa1 (toxB). Here we report for the first time that efa1 inhibits mitogen-activated proliferation of bovine peripheral blood lymphocytes by EHEC O103:H2, but that E. coli K-12 strains expressing the N-terminal and central portions of the protein lack activity. While a Shiga toxin-negative E. coli O157:H7 strain was shown to possess lymphostatin-like activity, deletion of efa1' or toxB, singly or in combination, failed to significantly relieve the inhibitory effect.

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Citations

Dec 7, 2011·Infection and Immunity·Mauricio J Farfan, Alfredo G Torres
Oct 30, 2009·Journal of Applied Microbiology·D DöpferA M Urdahl
Apr 30, 2013·Virulence·Analía Inés Etcheverría, Nora Lía Padola
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
Apr 20, 2017·Frontiers in Cellular and Infection Microbiology·Yanmei XuYanwen Xiong

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