Functional analysis of MADS-box genes controlling ovule development in Arabidopsis using the ethanol-inducible alc gene-expression system

Mechanisms of Development
Raffaella BattagliaMartin M Kater

Abstract

In Arabidopsis, different combinations of ABC organ identity proteins interact in the presence of SEPALLATA (SEP) proteins to regulate floral organ differentiation. Ectopic expression of SEP3 in combination with class A and B or B and C genes is sufficient to homeotically convert vegetative leaves into petal-like organs and bracts into stamen-like structures, respectively. Recently, it has been shown that the three MADS-box genes SEEDSTICK (STK), SHATTERPROOF1 (SHP1) and SHP2 act redundantly to control ovule identity. Protein interaction assays performed in yeast in combination with genetic studies demonstrated that these MADS-box factors only interact in the presence of SEP proteins to form complexes that determine ovule differentiation. Here, we address the question whether the ectopic co-expression of ovule identity proteins is sufficient to induce the homeotic conversion of vegetative leaves into carpel-like structures bearing ovules. We present the phenotypic characterization of Arabidopsis plants that ectopically express ovule identity factors under the regulation of the ethanol inducible gene expression system. These experiments indicate that the ectopic co-expression of SEP3 and SHP1 and/or STK is probably not sufficien...Continue Reading

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Citations

Jan 1, 2010·Plant Molecular Biology Reporter·Jiaohui XuHongyu Li
Sep 20, 2007·Science in China. Series C, Life Sciences·JinHui FanHuaiRui Shu
Feb 1, 2014·Journal of Experimental Botany·Sara Simonini, Martin M Kater
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Apr 12, 2014·Frontiers in Plant Science·Mara CucinottaIrma Roig-Villanova
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Mar 19, 2020·International Journal of Molecular Sciences·Xiao ZhangQibin Ma
Mar 12, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Meng-Xin LuZi-Qin Xu

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