Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin

Malaria Journal
Micheline GuillotteO Mercereau-Puijalon

Abstract

Rosetting, namely the capacity of the Plasmodium falciparum-infected red blood cells to bind uninfected RBCs, is commonly observed in African children with severe malaria. Rosetting results from specific interactions between a subset of variant P. falciparum erythrocyte membrane protein 1 (PfEMP1) adhesins encoded by var genes, serum components and RBC receptors. Rosette formation is a redundant phenotype, as there exists more than one var gene encoding a rosette-mediating PfEMP1 in each genome and hence a diverse array of underlying interactions. Moreover, field diversity creates a large panel of rosetting-associated serotypes and studies with human immune sera indicate that surface-reacting antibodies are essentially variant-specific. To gain better insight into the interactions involved in rosetting and map surface epitopes, a panel of monoclonal antibodies (mAbs) was investigated. Monoclonal antibodies were isolated from mice immunized with PfEMP1-VarO recombinant domains. They were characterized using ELISA and reactivity with the native PfEMP1-VarO adhesin on immunoblots of reduced and unreduced extracts, as well as SDS-extracts of Palo Alto 89F5 VarO schizonts. Functionality was assessed using inhibition of Palo Alto 89F...Continue Reading

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Citations

Nov 17, 2020·Frontiers in Immunology·S Jake GonzalesEvelien M Bunnik

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Datasets Mentioned

BETA
EU908205

Methods Mentioned

BETA
flow cytometry
ELISA
protein assay
flow
fluorescence microscopy
X-ray

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