Functional analysis of myelodysplastic syndromes-derived mesenchymal stem cells

Leukemia Research
E Flores-FigueroaHéctor Mayani

Abstract

Two different reports, including one from our own group, have recently demonstrated the presence of severe chromosomal abnormalities in mesenchymal stem cells (MSC) from patients with myelodysplastic syndromes (MDS). In the present study, we have assessed whether such cytogenetic abnormalities result in functional deficiencies in vitro. We found that both normal and MDS MSC showed similar expression patterns of cell adhesion molecules and extracellular matrix proteins. MDS MSC layers showed the capability to differentiate towards adipocytes, chondrocytes and osteoblasts, and supported the growth of early umbilical cord blood progenitors in a co-culture system. Unstimulated MDS MSC secreted more IL-1beta and after treatment with TNFalpha, they secreted more SCF, as compared to their normal counterparts. The present study demonstrates that, in spite of harboring severe chromosomal alterations, most of the functional properties of MDS-derived MSC remain normal, including their ability to support normal hematopoiesis in vitro.

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Mar 1, 1993·British Journal of Haematology·S OhmoriM Okuma
Apr 2, 1999·Science·M F PittengerD R Marshak
Dec 21, 2000·British Journal of Haematology·G B TennantA K Burnett
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Juan LiAnthony R Green
Dec 21, 2004·Leukemia Research·Eugenia Flores-FigueroaHector Mayani
Nov 10, 2006·Leukemia Research·Rosimeire A RoelaM Mitzi Brentani
Nov 23, 2006·Blood·Eugenia Flores-Figueroa, Hector Mayani
Oct 5, 2007·Leukemia·B HerrerosM A Piris
Nov 28, 2007·Annual Review of Pathology·Thea D Tlsty, Lisa M Coussens

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Citations

Mar 24, 2012·Bulletin of Experimental Biology and Medicine·O N LubkovaT E Manakova
Jan 31, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Pranela Rameshwar
Nov 9, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michaela R Reagan, Irene M Ghobrial
Dec 24, 2010·PloS One·Manoj M PillaiBeverly Torok-Storb
Jan 5, 2014·Clinical & Developmental Immunology·Maria-Christina KastrinakiHelen A Papadaki
Jan 21, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chengming FeiChunkang Chang
Dec 10, 2008·Expert Opinion on Biological Therapy·Aravind Ramakrishnan, H Joachim Deeg
Aug 8, 2015·Science China. Life Sciences·JingJing Xie, ChengCheng Zhang
Apr 15, 2011·British Journal of Haematology·Shikhar AggarwalHetty J Bontkes
Mar 31, 2015·British Journal of Haematology·Edita HamzicRuth Pettengell
Feb 11, 2015·Cellular and Molecular Life Sciences : CMLS·Lili YangSheng Wei
Nov 15, 2014·Leukemia·E BulychevaU Platzbecker
Apr 19, 2016·International Journal of Molecular Sciences·Eugenia Flores-Figueroa, Dita Gratzinger
Sep 24, 2016·Analytical Cellular Pathology (Amsterdam)·A J I S RathnayakeV H W Dissanayake
Nov 9, 2016·Critical Reviews in Oncology/hematology·Claudio FozzaFausto Dore
Jan 9, 2017·Blood·Claudia Korn, Simón Méndez-Ferrer
Jan 17, 2017·Blood Research·Thomas SchroederRainer Haas
Dec 10, 2017·Cellular and Molecular Life Sciences : CMLS·Marie GoulardDominique Bonnet

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