PMID: 7542658Aug 1, 1995Paper

Functional analysis of posttranslational cleavage products of the neuron-glia cell adhesion molecule, Ng-CAM

The Journal of Cell Biology
M P BurgoonB A Cunningham

Abstract

Neuron-glia cell adhesion molecule (Ng-CAM) mediates cell adhesion between neurons homophilically and between neurons and glia heterophilically; it also promotes neurite outgrowth. In the chick brain, Ng-CAM is detected as glycoproteins of 190 and 210 kD (Ng-CAM200) with posttranslational cleavage products of 135 kD (F135, which contains most of the extracellular region) and 80 kD (F80, which includes the transmembrane and the cytoplasmic domains). To examine the functions of each of these components, we have expressed Ng-CAM200, F135, and F80 in murine L cells, and F135 and F80 as GST fusion proteins in the pGEX vector in bacteria. Appropriately transfected L cells expressed each of these proteins on their surfaces; F135 was also found in the media of cells transfected with Ng-CAM200 and F135. In addition to binding homophilically, cells transfected with Ng-CAM200 and F135 bound heterophilically to untransfected L cells, suggesting that there is a ligand for Ng-CAM on fibroblasts that may be related to the glial ligand. Detailed studies using the transfected cells and the fusion proteins indicated that both the homophilic and the heterophilic binding activities of Ng-CAM are localized in the F135 fragment of the molecule. The ...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·K M YamadaS E LaFlamme
Nov 1, 1990·Journal of Neuroscience Research·J Schneider-SchauliesM Schachner
Feb 1, 1991·The Journal of Cell Biology·E T StoeckliP Sonderegger
Feb 1, 1990·Journal of Neuroscience Research·S ChangJ A Raper
Aug 1, 1986·The Journal of Cell Biology·F RiegerG M Edelman
Sep 1, 1986·The Journal of Cell Biology·J K DaniloffG M Edelman
Dec 1, 1988·The Journal of Cell Biology·D R FriedlanderG M Edelman
Jan 1, 1988·Annual Review of Biochemistry·E Ruoslahti
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L BixbyL F Reichardt
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C Lagenaur, V Lemmon
Jul 1, 1986·The Journal of Cell Biology·S HoffmanG M Edelman
Feb 1, 1987·The Journal of Cell Biology·F G RathjenU Rutishauser
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B A CunninghamG M Edelman
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M GrumetG M Edelman
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M GrumetG M Edelman
Sep 1, 1993·Cell Adhesion and Communication·M GrumetG M Edelman

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Citations

Feb 7, 1998·Journal of the Neurological Sciences·S M Chou, H S Wang
Oct 1, 1996·Current Opinion in Neurobiology·T Brümmendorf, F G Rathjen
Aug 7, 2014·Journal of the Neurological Sciences·Ling WangSi-Jie Qiao
Mar 20, 2010·Cellular and Molecular Life Sciences : CMLS·Michael K E Schäfer, Peter Altevogt
Dec 16, 1998·The Journal of Cell Biology·S KunzP Sonderegger
Mar 1, 1997·Cell Adhesion and Communication·R B HazanP I Borgen
Feb 1, 1997·Differentiation; Research in Biological Diversity·G Kadmon, P Altevogt

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