Functional Analysis of RNA Interference-Related Soybean Pod Borer (Lepidoptera ) Genes Based on Transcriptome Sequences

Frontiers in Physiology
Fanli MengWenbin Li

Abstract

RNA interference (RNAi) is useful for controlling pests of agriculturally important crops. The soybean pod borer (SPB) is the most important soybean pest in Northeastern Asia. In an earlier study, we confirmed that the SPB could be controlled via transgenic plant-mediated RNAi. Here, the SPB transcriptome was sequenced to identify RNAi-related genes, and also to establish an RNAi-of-RNAi assay system for evaluating genes involved in the SPB systemic RNAi response. The core RNAi genes, as well as genes potentially involved in double-stranded RNA (dsRNA) uptake were identified based on SPB transcriptome sequences. A phylogenetic analysis and the characterization of these core components as well as dsRNA uptake related genes revealed that they contain conserved domains essential for the RNAi pathway. The results of the RNAi-of-RNAi assay involving Laccase 2 (a critical cuticle pigmentation gene) as a marker showed that genes encoding the sid-like (Sil1), scavenger receptor class C (Src), and scavenger receptor class B (Srb3 and Srb4) proteins of the endocytic pathway were required for SPB cellular uptake of dsRNA. The SPB response was inferred to contain three functional small RNA pathways (i.e., miRNA, siRNA, and piRNA pathways)....Continue Reading

References

Mar 15, 2006·The Journal of Biological Chemistry·Johanna UlvilaMika Rämet
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·K KimR W Carthew
Oct 29, 2008·Molecular and Cellular Biology·Xiao-cui YangZbigniew Dominski
Oct 20, 2009·Journal of Insect Physiology·Hanneke Huvenne, Guy Smagghe
Dec 7, 2011·PloS One·Kyaw Min AungKozo Fujisaki
Sep 18, 2012·Current Biology : CB·Andrea HinasCraig P Hunter
Feb 5, 2013·Molecular Cell·Kazumichi M NishidaMikiko C Siomi
Aug 21, 2013·Transgenic Research·Jianjun Mao, Fanrong Zeng
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 8, 2014·Biological Chemistry·Anne Dueck, Gunter Meister
Dec 30, 2014·Journal of Insect Physiology·Chaoyang ZhaoOmprakash Mittapalli
Aug 30, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Azali AzlanGhows Azzam
Dec 3, 2016·Scientific Reports·Clauvis N T TaningGuy Smagghe
Dec 3, 2016·Frontiers in Physiology·Mallikarjuna R JogaOlivier Christiaens
Jan 8, 2017·Genome Biology and Evolution·Daniel DowlingOliver Niehuis

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Datasets Mentioned

BETA
SRR5985989

Methods Mentioned

BETA
transgenic

Software Mentioned

BLAST
Primer
ScanProsite
Multiple
TMHMM Server
Trinity
BLASTX
tBLASTn
MEGA

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