Functional analysis of slow myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
Jin XuShao Jun Du

Abstract

Myofibrillogenesis, the process of sarcomere formation, requires close interactions of sarcomeric proteins and various components of sarcomere structures. The myosin thick filaments and M-lines are two key components of the sarcomere. It has been suggested that myomesin proteins of M-lines interact with myosin and titin proteins and keep the thick and titin filaments in order. However, the function of myomesin in myofibrillogenesis and sarcomere organization remained largely enigmatic. No knockout or knockdown animal models have been reported to elucidate the role of myomesin in sarcomere organization in vivo. In this study, by using the gene-specific knockdown approach in zebrafish embryos, we carried out a loss-of-function analysis of myomesin-3 and slow myosin heavy chain 1 (smyhc1) expressed specifically in slow muscles. We demonstrated that knockdown of smyhc1 abolished the sarcomeric localization of myomesin-3 in slow muscles. In contrast, loss of myomesin-3 had no effect on the sarcomeric organization of thick and thin filaments as well as M- and Z-line structures. Together, these studies indicate that myosin thick filaments are required for M-line organization and M-line localization of myomesin-3. In contrast, myomesin...Continue Reading

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Citations

Sep 27, 2013·Molecular Biology of the Cell·Huiqing LiShao Jun Du
Jul 30, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zhiliang ChenShengyun Fang
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jingchun YangXiaolei Xu
Aug 28, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jianmeng CaoDeli Shi
Sep 12, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Paula Suarez-BreguaJosep Rotllant
Mar 1, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mengxin CaiShaojun Du
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Siping LiShaojun Du
Mar 11, 2021·International Journal of Biological Macromolecules·Huijuan LiQi Li
May 8, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Shuang JiaoShaojun Du
Nov 13, 2021·Frontiers in Cell and Developmental Biology·Rui Xu, Shaojun Du

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