Functional analysis of structural genes for NAD(+)-dependent formate dehydrogenase in Saccharomyces cerevisiae

Yeast
Karin M OverkampJack T Pronk

Abstract

Co-consumption of formate by aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae CEN.PK 113-7D led to an increased biomass yield relative to cultures grown on glucose as the sole carbon and energy substrate. In this respect, this strain differed from two previously investigated S. cerevisiae strains, in which formate oxidation did not lead to an increased biomass yield on glucose. Enzyme assays confirmed the presence of a formate-inducible, cytosolic and NAD(+)-dependent formate dehydrogenase. To investigate whether this enzyme activity was entirely encoded by the previously reported FDH1 gene, an fdh1Delta null mutant was constructed. This mutant strain still contained formate dehydrogenase activity and remained capable of co-consumption of formate. The formate dehydrogenase activity in the mutant was demonstrated to be encoded by a second structural gene for formate dehydrogenase (FDH2) in S. cerevisiae CEN.PK 113-7D. FDH2 was highly homologous to FDH1 and consisted of a fusion of two open reading frames (ORFs) (YPL275w and YPL276w) reported in the S. cerevisiae genome databases. Sequence analysis confirmed that, in the database genetic background, the presence of two single-nucleotide differences led to t...Continue Reading

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Citations

Feb 10, 2009·Applied and Environmental Microbiology·Zeev Waks, Pamela A Silver
Dec 22, 2009·Applied and Environmental Microbiology·Jin HouGoutham N Vemuri
Apr 2, 2008·Applied and Environmental Microbiology·Richard J S BaerendsJack T Pronk
Nov 13, 2012·Applied and Environmental Microbiology·Apostolos AlissandratosChristopher J Easton
Mar 7, 2013·Canadian Journal of Microbiology·Yogesh GoyalKalyan Gayen
Jul 1, 2009·Genetics·Caleb J KennedyPamela A Silver
Aug 8, 2008·Bioscience, Biotechnology, and Biochemistry·Yoshifumi MaedaHiroyuki Uchida
Jan 13, 2015·Current Opinion in Biotechnology·Andreas K Gombert, Antonius J A van Maris
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Oct 14, 2014·Journal of Industrial Microbiology & Biotechnology·Jiazhang Lian, Huimin Zhao
Dec 19, 2003·FEMS Microbiology Letters·Danilo PorroLilia Alberghina
Jul 28, 2016·Current Opinion in Chemical Biology·Oren YishaiArren Bar-Even
Jul 12, 2002·Yeast
Apr 5, 2003·The Journal of Biological Chemistry·Elton T YoungTrey Ideker
Jun 8, 2018·Journal of Industrial Microbiology & Biotechnology·Victor E Balderas-HernándezRadhakrishnan Mahadevan
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Apr 20, 2019·ACS Synthetic Biology·Jorge Gonzalez de la CruzArren Bar-Even

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