Apr 9, 2020

Room-temperature-storable PCR Mixes for SARS-CoV-2 Detection

BioRxiv : the Preprint Server for Biology
J. XuN. Xia

Abstract

A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] in Wuhan, China, which then rapidly spread globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks. Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration. We found that freeze-dried PCR mixes with ~1.2% residual moisture are optimal for storage, transport, and reconstitution. The sensitivity, specificity, and repeatability of the freeze-dried reagents were similar to those of freshly prepared, wet reagents. The freeze-dried mixes retained activity at room temperature (18~25) for 28 days, and for 14 and 10 days when stored at 37 and 56, respectively. The uptake of this approach will ease logistical challenges faced by transport ...Continue Reading

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Mentioned in this Paper

Study
Size
Viral Genome Location
Nodule
Virus
Genome
Genes
CRISPR-Cas Systems
Metagenome
Environment

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