PMID: 9525647Apr 3, 1998Paper

Functional analysis of the CAAT box in the major late promoter of the subgroup C human adenoviruses

Journal of Virology
B Song, C S H Young

Abstract

Comparisons among sequences predicted to encode the major late promoter (MLP) of adenoviruses from a wide variety of host species show that an inverted CAAT box is among the most highly conserved transcription elements found in the putative MLPs. The high degree of conservation suggests that the CAAT box plays an important role in the function of the MLP in vivo, an idea supported by a previous mutational analysis of the core CCAAT sequence. To address the importance of the CAAT box, in terms both of quantitative levels of transcription and of specificity, a further set of mutations was created and examined in the context of the viral genome. One mutation, CAAT5, contains individual changes at five positions, four of which correspond to invariant residues in a CAAT box consensus derived either by computer analysis or empirically. The CAAT5 mutation had no discernible phenotype by itself but when coupled with the previously described USF0 mutation, which disrupts binding of the upstream stimulating factor (USF) but is otherwise phenotypically silent, gave rise to virus with a severe replication deficiency. Nuclear run-on assays showed that transcription initiation at the mutant MLP was significantly reduced compared with that of...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S N MaityB de Crombrugghe
Jan 1, 1990·Molecular and Cellular Biology·I C Taylor, R E Kingston
Apr 7, 1989·Cell·S T Smale, D Baltimore
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y S Lin, M R Green
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K A LeeM R Green
Jan 1, 1986·Annual Review of Genetics·A J Berk
Apr 8, 1988·Cell·L A ChodoshP A Sharp
Jul 29, 1988·Science·S N MaityB de Crombrugghe
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Nov 1, 1973·Journal of the National Cancer Institute·D J GiardW P Parks
Oct 1, 1967·Journal of Virology·W C Lawrence, H S Ginsberg
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 11, 1984·Nucleic Acids Research·M E MulliganW R McClure
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S SinhaB de Crombrugghe
Apr 1, 1997·The Journal of General Virology·M D MorrisonL Nicolson
Oct 23, 1997·The Journal of Biological Chemistry·W BiS N Maity

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Citations

Mar 2, 2013·Cell Death and Differentiation·D Dolfini, R Mantovani
Feb 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marie C ChiaFei-Fei Liu

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