PMID: 9533959May 12, 1998Paper

Functional analysis of the catalytic subunit of Dictyostelium PKA in vivo

Mechanisms of Development
H DammannM Veron

Abstract

The catalytic subunit of the cAMP-dependent protein kinase (PKA) from Dictyostelium discoideum contains several domains, including an unusually long N-terminal extension preceding a highly conserved catalytic core. We transformed the aggregationless PkaC-null strain with several deletion constructs of both domains. Strains transformed with genes expressing catalytically-inactive polypeptides could not rescue development. Cotransformation with constructs encoding the N-terminal extension and the catalytic core, both unable to rescue development by themselves, yielded transformants able to proceed to late development. A 27-amino acid long hydrophobic region, immediately upstream of the catalytic core, was found indispensable for PKA function. A putative role of this sequence in the acquisition of the active conformation of the protein is discussed.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 15, 1992·Biochemical and Biophysical Research Communications·M N SimonJ C da Costa Maia
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K MannR A Firtel
Jan 1, 1992·Developmental Biology·A J HarwoodJ G Williams
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J D ScottE G Krebs
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R MutzelM Veron
Jan 1, 1987·Methods in Cell Biology·W NellenR A Firtel
Oct 12, 1987·Nucleic Acids Research·C D Reymond
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Sep 1, 1970·The Biochemical Journal·D J Watts, J M Ashworth
Jan 1, 1983·Methods in Enzymology·R Roskoski
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M VeronS S Taylor
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K StottM Perutz
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·S K MannR A Firtel
May 15, 1994·Structure·S S Taylor, E Radzio-Andzelm
Oct 1, 1993·Protein Science : a Publication of the Protein Society·J ZhengL F Ten Eyck
Mar 1, 1993·Trends in Biochemical Sciences·S S TaylorM J Zoller
Dec 18, 1995·Experientia·C D ReymondJ G Williams
Jan 1, 1996·Annual Review of Biochemistry·C A Parent, P N Devreotes
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·G ShaulskyW F Loomis
Feb 13, 1997·Nature·F SicheriJ Kuriyan

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