Functional analysis of the galactosyltransferases required for biosynthesis of D-galactan I, a component of the lipopolysaccharide O1 antigen of Klebsiella pneumoniae

Journal of Bacteriology
S GuanC Whitfield

Abstract

D-Galactan I is an O-antigenic polymer with the repeat unit structure [-->3)-beta-D-Galf-(1-->3)-alpha-D-Galp-(1-->], that is found in the lipopolysaccharide of Klebsiella pneumoniae O1 and other gram-negative bacteria. A genetic locus containing six genes is responsible for the synthesis and assembly of D-galactan I via an ATP-binding cassette (ABC) transporter-dependent pathway. The galactosyltransferase activities that are required for the processive polymerization of D-galactan I were identified by using in vitro reactions. The activities were determined with endogenous lipid acceptors in membrane preparations from Escherichia coli K-12 expressing individual enzymes (or combinations of enzymes) or in membranes reconstituted with specific lipid acceptors. The D-galactan I polymer is built on a lipid acceptor, undecaprenyl pyrophosphoryl-GlcpNAc, a product of the WecA enzyme that participates in the biosynthesis of enterobacterial common antigen and O-antigenic polysaccharide (O-PS) biosynthesis pathways. This intermediate is directed into D-galactan I biosynthesis by the bifunctional wbbO gene product, which sequentially adds one Galp and one Galf residue from the corresponding UDP-sugars to form a lipid-linked trisaccharide...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1991·Molecular Microbiology·M OhtaN Kato
Jun 1, 1991·Canadian Journal of Microbiology·J BinottoK E Sanderson
Sep 1, 1988·FEMS Microbiology Reviews·H M KuhnH Mayer
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·N H Behrens, L F Leloir
Sep 1, 1982·European Journal of Biochemistry·K JannS Kanegasaki
Jan 1, 1994·Microbiology·D Liu, P R Reeves
May 1, 1995·Trends in Microbiology·C Whitfield
Nov 8, 1996·The Journal of Biological Chemistry·W J Keenleyside, C Whitfield
Sep 25, 1997·Clinical and Diagnostic Laboratory Immunology·M TrautmannC Whitfield

❮ Previous
Next ❯

Citations

Jul 18, 2002·The Journal of Experimental Medicine·Asesh BanerjeePeter A Rice
Jun 19, 2002·Glycobiology·Laure JollyFrancesca Stingele
Sep 24, 2011·Glycobiology·Boris TefsenFrançoise H Routier
Sep 27, 2002·Applied and Environmental Microbiology·Christina SchäfferChris Whitfield
Jun 17, 2008·Eukaryotic Cell·Philipp S SchmalhorstFrançoise H Routier
Feb 24, 2004·Infection and Immunity·Sunita Shankar-SinhaJohn G Younger
Sep 6, 2006·Journal of Bacteriology·Katarína MikusováPatrick J Brennan
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Oct 7, 2005·Biological Chemistry·Hans BakkerFrançoise H Routier
Jul 16, 2013·FEMS Microbiology Reviews·Bin LiuLei Wang
Nov 28, 2008·The Journal of Biological Chemistry·Veronica KosChris Whitfield
Jan 25, 2017·Antimicrobial Agents and Chemotherapy·Frank R DeLeoBarry N Kreiswirth
Jul 11, 2009·Chembiochem : a European Journal of Chemical Biology·Michele R Richards, Todd L Lowary
Jun 23, 2020·Annual Review of Biochemistry·Christopher A CaffaletteJochen Zimmer
Jan 16, 2018·Bioscience, Biotechnology, and Biochemistry·Takuji Oka
Nov 6, 2009·The Journal of Biological Chemistry·Myles B PoulinTodd L Lowary
Apr 1, 2018·The Journal of Biological Chemistry·Bradley R ClarkeChris Whitfield
Aug 29, 2019·MBio·Yara SeifBernhard O Palsson
Mar 11, 2020·Nature Chemical Biology·Bradley R ClarkeChris Whitfield
Nov 30, 2019·Frontiers in Cellular and Infection Microbiology·L Ponoop Prasad Patro, Thenmalarchelvi Rathinavelan
Sep 24, 2002·The Journal of Biological Chemistry·Hanke Van Der WelChristopher M West
May 20, 2020·International Journal of Molecular Sciences·Mateja SeničarRichard Daniellou
Nov 22, 2019·ACS Chemical Biology·Liubov Yakovlieva, Marthe T C Walvoort
Apr 6, 2012·Carbohydrate Research·Laura K Greenfield, Chris Whitfield
Nov 29, 2007·Bioorganic & Medicinal Chemistry Letters·Inka BrockhausenOle Hindsgaul

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.