Functional analysis of the gene encoding immunity to lactacin F, lafI, and its use as a Lactobacillus-specific, food-grade genetic marker.

Applied and Environmental Microbiology
G E Allison, T R Klaenhammer

Abstract

Lactacin F is a two-component class II bacteriocin produced by Lactobacillus johnsonii VPI 11088. The laf operon is composed of the bacteriocin structural genes, lafA and lafX, and a third open reading frame, ORFZ. Two strategies were employed to study the function of ORFZ. This gene was disrupted in the chromosome of NCK64, a lafA729 lafX ORFZ derivative of VPI 11088. A disruption cassette consisting of ORFZ interrupted with a cat gene was cloned into pSA3 and introduced into NCK64. Manipulation of growth temperatures and antibiotic selection resulted in homologous recombination which disrupted the chromosomal copy of ORFZ with the cat gene. This ORFZ mutation resulted in loss of immunity to lactacin F but had little effect on production of LafX, which is not bactericidal without LafA. Expression of ORFZ in this ORFZ- background rescued the immune phenotype. Expression of ORFZ in a bacteriocin-sensitive derivative of VPI 11088 also reestablished immunity. These data indicate that ORFZ, renamed lafI, encodes the immunity factor for the lactacin F system. The sensitivity of various Lactobacillus strains to lactacin F was further evaluated. Lactacin F inhibited 11 strains including several members of the A1, A2, A3, A4, B1, and B...Continue Reading

References

Feb 1, 1992·Gene·P Le BourgeoisP Ritzenthaler
Feb 1, 1991·Applied and Environmental Microbiology·B BojovicL Topisirovic
Feb 1, 1991·Applied and Environmental Microbiology·M J van BelkumG Venema
Oct 1, 1987·Applied and Environmental Microbiology·J M van der VossenG Venema
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Jan 1, 1985·Applied and Environmental Microbiology·M L Dao, J J Ferretti
Jun 1, 1983·Applied and Environmental Microbiology·S F Barefoot, T R Klaenhammer
Oct 15, 1984·Journal of Molecular Biology·D EisenbergR Wall
Oct 1, 1995·Applied and Environmental Microbiology·M J van Belkum, M E Stiles
Nov 1, 1994·Molecular Microbiology·K VenemaJ Kok
Jul 1, 1995·Microbiology·C FremauxY Cenatiempo
May 1, 1995·Applied Microbiology and Biotechnology·E WankerH Schwab
Jun 1, 1995·Journal of Bacteriology·R W WoroboM E Stiles
Jan 1, 1993·Antonie van Leeuwenhoek·P H Pouwels, R J Leer
Oct 1, 1993·Journal of Bacteriology·T BhowmikJ L Steele
Nov 1, 1993·Applied and Environmental Microbiology·C FremauxT R Klaenhammer
Feb 1, 1996·Journal of Bacteriology·G MollA J Driessen
Feb 1, 1996·Antonie van Leeuwenhoek·P E SarisH G Sahl
Feb 1, 1996·Antonie van Leeuwenhoek·J Delves-BroughtonJ Hugenholtz
May 1, 1996·FEMS Microbiology Letters·D C WalkerT R Klaenhammer
Apr 1, 1993·Applied and Environmental Microbiology·K VenemaG Venema
Sep 1, 1993·Applied and Environmental Microbiology·M E Bruno, T J Montville
Apr 1, 1996·Applied and Environmental Microbiology·H Joosten, M Nunez

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Citations

May 1, 2013·Critical Reviews in Food Science and Nutrition·Paulraj KanmaniVenkatesan Arul
Jan 30, 2020·Biotechnology Journal·Sung Won ChoSang Woo Seo
Feb 9, 2000·Journal of Applied Microbiology·M ZamfirL De Vuyst
Jun 1, 2000·Applied and Environmental Microbiology·M C MartínM A Alvarez
Nov 19, 2019·Journal, Genetic Engineering & Biotechnology·Tejinder KaurBaljinder Kaur
Jan 7, 2003·Applied and Environmental Microbiology·Paul D CotterR Paul Ross
Aug 23, 2011·Biotechnology Journal·Clemens PeterbauerDietmar Haltrich
Nov 26, 2013·Applied and Environmental Microbiology·Tyler E AskelsonTri Duong
Jun 6, 2016·World Journal of Microbiology & Biotechnology·José M LandeteJuan L Arqués
Aug 7, 2003·Applied and Environmental Microbiology·Hassan A M El DemerdashArnold Geis
Apr 26, 2008·FEMS Microbiology Letters·Raymond David PridmoreChristoph Cavadini
Jan 1, 2016·Gut Pathogens·Senait AssefaGerwald A Köhler
Feb 27, 2016·Critical Reviews in Biotechnology·José Maria Landete
Aug 8, 2017·International Journal of Molecular Sciences·José María Landete, Juan Luis Arqués
Feb 7, 2001·The American Journal of Clinical Nutrition·C StantonR P Ross
Aug 2, 2003·FEMS Microbiology Letters·Soo Jin JangDae Kyun Chung
Dec 2, 2017·Biotechnology & Genetic Engineering Reviews·Daniel Bravo, José M Landete
Nov 9, 2002·Biochimie·Sylvie GarneauJohn C Vederas
Dec 29, 2005·Biotechnology Advances·Xu Xia ZhouYuan Jiang Pan
Apr 21, 2011·Journal of Agricultural and Food Chemistry·Tien-Thanh NguyenClemens K Peterbauer

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