Functional analysis of the homoserine O-acetyltransferase gene and its identification as a selectable marker in Gibberella zeae

Current Genetics
You-Kyoung HanYin-Won Lee

Abstract

We used restriction enzyme-mediated integration (REMI) to identify a methionine auxotrophic mutant of Gibberella zeae, an important cereal pathogen. In addition to its methionine requirement, the G. zeae REMI mutant designated Z43R3912 showed pleiotropic phenotypes, including reduced virulence on host plants and lack of sexual development. Outcrossing of Z43R3912 with a mat1-1 deletion strain confirmed that the mutation of Z43R3912 was tagged with the hygromycin B resistance marker. The vector insertion site in Z43R3912 was identified within the ORF designated GzmetE, encoding a putative homoserine O-acetyltrasferase (HOA). Gene disruption analyses confirmed that GzmetE was responsible for the pleiotropic phenotypes of Z43R3912. Genetic complementation of the G. zeae methionine auxotroph with an intact copy of the Aspergillus nidulans metE and GzmetE genes suggests that the HOA gene can be used as a selectable marker for transformation of G. zeae.

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Citations

Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Won-Bo ShimYin-Won Lee
Nov 8, 2013·World Journal of Microbiology & Biotechnology·Jing FuZhonghua Ma
Jan 18, 2011·Fungal Genetics and Biology : FG & B·Hokyoung SonYin-Won Lee
Sep 15, 2010·Biochemical and Biophysical Research Communications·Jungkwan LeeYin-Won Lee
Jan 28, 2010·Fungal Genetics and Biology : FG & B·Sae-Yeon HongYin-Won Lee
Nov 20, 2014·Amino Acids·Kamila Jastrzębowska, Iwona Gabriel
May 30, 2019·Current Genetics·Mikkel Rank NielsenJens Laurids Sørensen
Mar 23, 2019·Frontiers in Microbiology·Xinyue FanHansong Dong

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