PMID: 9525614Apr 3, 1998Paper

Functional analysis of the human immunodeficiency virus type 1 Rev protein oligomerization interface

Journal of Virology
S L ThomasJ Hauber

Abstract

The expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the action of the viral trans-regulatory protein Rev. Rev is a nuclear shuttle protein that directly binds to its cis-acting Rev response element (RRE) RNA target sequence. Subsequent oligomerization of Rev monomers on the RRE and interaction of Rev with a cellular cofactor(s) result in the cytoplasmic accumulation of RRE-containing viral mRNAs. Moreover, Rev by itself is exported from the nucleus to the cytoplasm. Although it has been demonstrated that Rev multimerization is critically required for Rev activity and hence for HIV-1 replication, the number of Rev monomers required to form a trans-activation-competent complex on the RRE is unknown. Here we report a systematic analysis of the putative multimerization domains within the Rev trans-activator protein. We identify the amino acid residues which are part of the proposed single hydrophobic surface patch in the Rev amino terminus that mediates intermolecular interactions. Furthermore, we show that the expression of a multimerization-deficient Rev mutant blocks HIV-1 replication in a trans-dominant (dominant-negative) fashion.

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Citations

Nov 18, 2016·Journal of Virology·Ryan T BehrensNathan M Sherer
Apr 29, 1999·Nucleic Acids Research·P C BriceP J Butler
Apr 15, 2000·The Journal of Biological Chemistry·O RosoriusJ Hauber
Jun 30, 2005·Animal Health Research Reviews·Marie-Claude St-LouisDenis Archambault
May 18, 2006·The Journal of Biological Chemistry·Marc ArnoldRalph H Kehlenbach
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael A DiMattiaJonathan M Grimes
Sep 1, 2006·Current Opinion in HIV and AIDS·Dirk Daelemans, Christophe Pannecouque
Aug 6, 2004·The Journal of Biological Chemistry·Dirk DaelemansGeorge N Pavlakis
Jan 26, 2008·Protein Science : a Publication of the Protein Society·Stephen P EdgcombMirko Hennig
Apr 16, 2005·The Journal of General Virology·Roochi Trikha, David W Brighty
Oct 9, 2002·Medicinal Research Reviews·Elena A LesnikDavid J Ecker
Aug 13, 2003·The Journal of Biological Chemistry·Bettina HartliebStephan Becker
Dec 29, 2005·AIDS Research and Human Retroviruses·Rajesh RamakrishnanNafees Ahmad
Jan 6, 1999·Virology·R H Stauber, G N Pavlakis
Jun 14, 2005·Retrovirology·Clemens FurnesAnne Marie Szilvay

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