Abstract
Lactobacillus fermentum temperate bacteriophage φPYB5 uses endolysin Lyb5 and holin Hyb5 to burst the host cell. Previous results showed that expression of Lyb5 in Escherichia coli caused host cell lysis slowly, leading us to suppose that Lyb5 could pass the cytoplasmic membrane partly. In this work, the function of a putative signal peptide (SPLyb5) at the N-terminal of Lyb5 was investigated. In E. coli, the cell adopted a spherical shape during induction of Lyb5 protein, while morphological changes were not observed during expression of the SPLyb5 truncation, indicating that the SPLyb5 motif may serve as a functional signal peptide. However, SPLyb5 was not proteolytically cleaved at the predicted site during the translocation of Lyb5, and the expressed Lyb5 protein appeared in the cytoplasm, cytoplasmic membrane and periplasm fractions with the same molecular mass. Similar results were obtained using Lactococcus lactis as a host to express Lyb5. These results indicated that SPLyb5 could direct Lyb5 to the periplasm in a membrane-tethered form, and then release it as a soluble active enzyme into the periplasm. In addition, SPLyb5 could also drive the fused NucleaseB protein to the extracytoplasm environment in E. coli as well ...Continue Reading
References
Oct 23, 1997·Nature Biotechnology·P G de RuyterW M de Vos
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 27, 2000·Journal of Bacteriology·C São-JoséM A Santos
Oct 6, 2000·Annual Review of Microbiology·I N WangR Young
Oct 13, 2000·Journal of Bacteriology·A GründlingR Young
Sep 7, 2001·Annual Review of Microbiology·H Brussow
Dec 3, 2002·Gene·Makiko KakikawaKen-Ichi Kodaira
Jun 12, 2003·Research in Microbiology·Roger W HendrixMargaret C M Smith
Aug 2, 2003·FEMS Microbiology Letters·Luis G Bermúdez-HumaránGérard Corthier
Apr 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Min XuRy Young
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic
Jan 8, 2005·Science·Min XuRy Young
Sep 14, 2006·Journal of Applied Microbiology·X ZhangY Qu
Nov 23, 2006·Trends in Biotechnology·Nicola K PettyGeorge P C Salmond
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Taehyun ParkRy Young
Oct 5, 2007·Journal of Biosciences·K V Srividhya, S Krishnaswamy
Nov 6, 2007·Journal of Bacteriology·João Gil NascimentoMário Almeida Santos
Feb 23, 2010·Applied and Environmental Microbiology·Shumin HuMingjie Ji
Jun 16, 2010·Molecular Microbiology·Maria João CatalãoMadalena Pimentel
Sep 14, 2010·Journal of Bacteriology·Gabriel F KutyRy Young
Nov 26, 2010·Current Opinion in Biotechnology·Lien CallewaertRob Lavigne
Dec 7, 2010·International Journal of Food Microbiology·Diego J MercantiAndrea Quiberoni
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Rebecca WhiteRy Young
Jun 21, 2011·Bacteriophage·Yves BriersRob Lavigne
Oct 26, 2011·Microbial Cell Factories·Josiane E Garneau, Sylvain Moineau
Mar 20, 2012·Applied and Environmental Microbiology·Melinda J MayerArjan Narbad
Dec 19, 2012·Annual Review of Food Science and Technology·Julie E Samson, Sylvain Moineau
Feb 15, 2013·Journal of Virology·Hugo OliveiraLeon D Kluskens
Oct 12, 2013·Current Opinion in Microbiology·Ry Young
Citations
Feb 24, 2016·Frontiers in Microbiology·Clara RocesBeatriz Martínez
May 16, 2013·PloS One·Shuguang LuFuquan Hu
Jan 7, 2018·Applied and Environmental Microbiology·Tingting GuoJian Kong
Jul 26, 2017·Science Progress·Jude AjueborAidan Coffey
Aug 1, 2018·Viruses·Sofia Fernandes, Carlos São-José
Aug 13, 2021·The Journal of Microbiology·Jun-Hyeok YuJong-Hyun Park