PMID: 9440507Jan 24, 1998Paper

Functional analysis of the two-gene lysis system of the pneumococcal phage Cp-1 in homologous and heterologous host cells

Journal of Bacteriology
A C MartínP Garcia

Abstract

The two lysis genes cph1 and cpl1 of the Streptococcus pneumoniae bacteriophage Cp-1 coding for holin and lysozyme, respectively, have been cloned and expressed in Escherichia coli. Synthesis of the Cph1 holin resulted in bacterial cell death but not lysis. The cph1 gene was able to complement a lambda Sam mutation in the nonsuppressing E. coli HB101 strain to produce phage progeny, suggesting that the holins encoded by both phage genes have analogous functions and that the pneumococcal holin induces a nonspecific lesion in the cytoplasmic membrane. Concomitant expression of both holin and lysin of Cp-1 in E. coli resulted in cell lysis, apparently due to the ability of the Cpl1 lysozyme to hydrolyze the peptidoglycan layer of this bacterium. The functional analysis of the cph1 and cpl1 genes cloned in a pneumococcal mutant with a complete deletion of the lytA gene, which codes for the S. pneumoniae main autolysin, provided the first direct evidence that, in this gram-positive-bacterium system, the Cpl1 endolysin is released to its murein substrate through the activity of the Cph1 holin. Demonstration of holin function was achieved by proving the release of pneumolysin to the periplasmic fraction, which strongly suggested that ...Continue Reading

References

Sep 1, 1992·Microbiological Reviews·R Young
Jul 15, 1986·European Journal of Biochemistry·J M Sanchez-PuellesE Garcia
Feb 1, 1996·Journal of Bacteriology·C SchmidtW Arber
Jul 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·R LópezJ L García
Apr 22, 1960·Biochimica Et Biophysica Acta·S LACKS, R D HOTCHKISS

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Citations

Jul 8, 2009·Journal of Bacteriology·Maria João FriasMário Ramirez
Oct 6, 2000·Annual Review of Microbiology·I N WangR Young
Sep 26, 2008·Current Microbiology·Jarmila Farkasovská, Andrej Godány
Aug 1, 2008·The Journal of Biological Chemistry·Begoña MonterrosoMargarita Menéndez

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