PMID: 9191025May 1, 1997Paper

Functional analysis of the yeast 40 kDa cyclophilin Cyp40 and its role for viability and steroid receptor regulation

Biological Chemistry
R WarthDidier Picard

Abstract

We have identified and characterized a homolog of the 40 kDa cyclophilins in the budding yeast Saccharomyces cerevisiae. At the amino acid level, this novel yeast cyclophilin, termed Cyp40, is 47% identical to human cyclophilin-40. Recombinant Cyp40 produced in bacteria has a peptidyl-prolyl cis-trans isomerase activity with a catalytic efficiency (k[cat]/K[m]) of 0.5 x 10(6)M(-1)s(-1), which can be inhibited by cyclosporin A with an IC50 value of 60nM. Using a polyclonal antibody against Cyp40 we have found that Cyp40 is predominantly cytoplasmic, and that its expression is induced 3-4-fold by heat shock. Moreover, Cyp40 can be coprecipitated from yeast extracts with the cytosolic molecular chaperone Hsp90. Surprisingly, a Cyp40-deficient yeast strain is fully viable at normal and elevated temperatures. Cyp40 is also dispensable for normal regulation of vertebrate steroid receptors in yeast. While other immunophilins could conceivably compensate a Cyp40 defect, our results are compatible with the notion that immunophilins may be fortuitous partners in the biochemically established steroid receptor-Hsp90 complex.

References

Jan 1, 1992·Annual Review of Immunology·N H Sigal, F J Dumont
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E S DavisM B Brennan
Jan 1, 1991·Methods in Enzymology·M SchenaK R Yamamoto
May 15, 1991·Biochemical and Biophysical Research Communications·M J BossardM A Levy
Nov 23, 1984·Biochimica Et Biophysica Acta·G FischerA Schellenberger
Jan 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·J M RenoirE E Baulieu
Apr 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A FrumanB E Bierer
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·T BreuderJ Heitman
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·A MatouschekG Schatz
Mar 1, 1995·Molecular and Biochemical Parasitology·G PalmerD Picard
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K SykesJ Sambrook
Sep 1, 1993·Trends in Biochemical Sciences·J Kunz, M N Hall
Apr 1, 1995·The Biochemical Journal·K Hoffmann, R E Handschumacher
Apr 6, 1995·Biochemical and Biophysical Research Communications·T RatajczakR F Minchin
Jan 13, 1995·The Journal of Biological Chemistry·G KernG Fischer
Nov 24, 1994·Nature·E K FrankeJ Luban
Sep 5, 1993·Journal of Molecular Biology·G Frigerio, H R Pelham
Sep 15, 1993·European Journal of Biochemistry·A Galat
Dec 1, 1995·Molecular and Cellular Biology·D F SmithR A Rimerman
Sep 1, 1995·Current Biology : CB·F X Schmid
Mar 7, 1996·Biochemical and Biophysical Research Communications·T RatajczakR F Minchin
May 31, 1996·The Journal of Biological Chemistry·K D DittmarW B Pratt
Jul 1, 1996·Physiological Reviews·A R Marks
Dec 6, 1996·Science·S BoseJ Buchner
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J F LouvionD Picard
Jan 1, 1997·Molecular and Cellular Biology·H C ChangS Lindquist
Sep 1, 1992·Trends in Cell Biology·M A StamnesC S Zuker

❮ Previous
Next ❯

Citations

Mar 16, 2000·Breast Cancer Research and Treatment·B K WardT Ratajczak
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·K DolinskiJ Heitman
Nov 5, 1998·Molecular Biology of the Cell·J F LouvionD Picard
Jan 27, 2004·Molecular Biology of the Cell·Paul LeeAvrom J Caplan
Mar 11, 2000·Microbiology and Immunology·R KanoA Hasegawa
Jan 9, 2004·Applied and Environmental Microbiology·Jaimie M Warren, Sarah F Covert
Oct 18, 2001·Molecular and Cellular Biology·T Abbas-TerkiD Picard
Aug 19, 2008·FEBS Letters·Tony C LuuWilliam K Chan
Oct 10, 2013·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Özlem Tastan BishopGregory Lloyd Blatch
Dec 17, 2009·Biochimica Et Biophysica Acta·Pablo C Echeverria, Didier Picard
Nov 20, 2002·Biological Chemistry·Toufik Abbas-TerkiDidier Picard
Sep 1, 2005·Yeast·Trevor J Pemberton, John E Kay
Jun 25, 1998·Molecular and Cellular Biology·Y FangA J Caplan
Mar 27, 2004·The Journal of Biological Chemistry·Otmar HainzlJohannes Buchner
Jun 6, 2003·The Journal of Biological Chemistry·Marija TesicRichard F Gaber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.