Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger

Archives of Microbiology
Mark ArentshorstArthur F J Ram

Abstract

Galactofuranose (Galf)-containing glycostructures are important to secure the integrity of the fungal cell wall. Golgi-localized Galf-transferases (Gfs) have been identified in Aspergillus nidulans and Aspergillus fumigatus. BLASTp searches identified three putative Galf-transferases in Aspergillus niger. Phylogenetic analysis showed that they group in three distinct groups. Characterization of the three Galf-transferases in A. niger by constructing single, double, and triple mutants revealed that gfsA is most important for Galf biosynthesis. The growth phenotypes of the ΔgfsA mutant are less severe than that of the ΔgfsAC mutant, indicating that GfsA and GfsC have redundant functions. Deletion of gfsB did not result in any growth defect and combining ΔgfsB with other deletion mutants did not exacerbate the growth phenotype. RT-qPCR experiments showed that induction of the agsA gene was higher in the ΔgfsAC and ΔgfsABC compared to the single mutants, indicating a severe cell wall stress response after multiple gfs gene deletions.

References

Jan 1, 1992·Methods in Enzymology·P J Punt, C A van den Hondel
Nov 24, 1999·Biochimica Et Biophysica Acta·G L WallisJ F Peberdy
Jun 17, 2008·Eukaryotic Cell·Philipp S SchmalhorstFrançoise H Routier
Jul 1, 2009·Cellular Microbiology·Claude LamarreJean-Paul Latgé
Oct 21, 2009·The Journal of Biological Chemistry·Jakob EngelFrançoise H Routier
May 24, 2011·International Journal of Medical Microbiology : IJMM·Leonie HeesemannFrank Ebel
Sep 24, 2011·Glycobiology·Boris TefsenFrançoise H Routier
Dec 21, 2011·Methods in Molecular Biology·Mark ArentshorstVera Meyer
Jan 16, 2018·Bioscience, Biotechnology, and Biochemistry·Takuji Oka

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
galactotransferase
dot-blot

Software Mentioned

Bio
DNAman2
Primer
BLAST
Rad CFX Manager

Related Concepts

Related Feeds

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.