Abstract
Mycobacterium tuberculosis (Mtb) is the causal agent of tuberculosis, the second largest infectious disease. With the rise of multi-drug resistant strains of M. tuberculosis, serious challenge lies ahead of us in treating the disease. The availability of complete genome sequence of Mtb has improved the scope for identifying new proteins that would not only further our understanding of biology of the organism but could also serve to discover new drug targets. In this study, Rv2345, a hypothetical membrane protein of M. tuberculosis H37Rv, which is reported to be a putative ortholog of ZipA cell division protein has been assigned function through functional annotation using bioinformatics tools followed by experimental validation. Sequence analysis showed Rv2345 to have a TPM domain at its N-terminal region and predicted it to have phosphatase activity. The TPM domain containing region of Rv2345 was cloned and expressed using pET28a vector in Escherichia coli and purified by Nickel affinity chromatography. The purified TPM domain was tested in vitro and our results confirmed it to have phosphatase activity. The enzyme activity was first checked and optimized with pNPP as substrate, followed by using ATP, which was also found to b...Continue Reading
References
Nov 11, 1994·Nucleic Acids Research·J D ThompsonT J Gibson
Jan 1, 1997·Annual Review of Biochemistry·J Lutkenhaus, S G Addinall
Jun 19, 2002·Biochemistry·Yasuhiro KashinoHimadri B Pakrasi
May 11, 2004·American Journal of Respiratory and Critical Care Medicine·David E Griffith
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
May 30, 2006·Bioinformatics·Weizhong Li, Adam Godzik
Jun 1, 2006·Microbiology·Richard A SlaydenJohn T Belisle
Jun 20, 2007·The Biochemical Journal·Sari SirpiöEva-Mari Aro
Nov 24, 2007·BMC Bioinformatics·Daniel H HusonRegula Rupp
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Erik C Hett, Eric J Rubin
Mar 8, 2008·Nature Reviews. Drug Discovery·Rowena L Lock, Elizabeth J Harry
May 15, 2008·BMC Structural Biology·Carolina Perez-Iratxeta, Miguel A Andrade-Navarro
Apr 13, 2011·PloS One·Edine W TiemersmaNico J D Nagelkerke
Jun 10, 2011·Molecular Plant·Kimberly M WegenerHimadri B Pakrasi
Sep 13, 2011·Plant Physiology·Hsin-Yi WuYi-Sheng Cheng
Dec 23, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Zarir F UdwadiaCamilla Rodrigues
Dec 27, 2011·Journal of Structural and Functional Genomics·Alexander EletskyThomas Szyperski
Aug 28, 2012·Nature Neuroscience·Thomas BoulinJean-Louis Bessereau
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Jan 10, 2014·ChemMedChem·Jaime L StarkRobert Powers
May 2, 2014·Frontiers in Microbiology·Catriona Donovan, Marc Bramkamp
Aug 15, 2014·Proteins·Martin AranDaniel O Cicero