Functional Analysis of Two Flavanone-3-Hydroxylase Genes from Camellia sinensis: A Critical Role in Flavonoid Accumulation

Genes
Yahui HanTao Xia

Abstract

Flavonoids are major secondary metabolites in Camellia sinensis. Flavanone-3-hydroxylase (F3H) is a key enzyme in flavonoid biosynthesis in plants. However, its role in the flavonoid metabolism in C. sinensis has not been well studied. In this study, we cloned two F3Hs from C. sinensis, named CsF3Ha and CsF3Hb, where CsF3Ha containing 1107 bases encoded 368 amino acids, and CsF3Hb containing 1071 bases encoded 357 amino acids. Enzymatic activity analysis showed both recombinant CsF3H enzymes in Escherichia coli could convert naringenin and eriodictyol into dihydrokaempferol (DHK) and dihydroquercetin (DHQ), respectively. The expression profiles showed that CsF3Ha and CsF3Hb were highly expressed in the tender leaves of tea plants. Under different abiotic stresses, the two CsF3Hs were induced remarkably by ultraviolet (UV) radiation, sucrose, and abscisic acid (ABA). In the seeds of CsF3Hs transgenic Arabidopsis thaliana, the concentration of most flavonol glycosides and oligomeric proanthocyanidins increased significantly, while the content of monocatechin derivatives decreased. The present study revealed that CsF3Hs played critical roles in flavonoid biosynthesis in tea plants.

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Citations

Apr 4, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min YangXiuqiao Zhang
Jan 8, 2020·Horticulture Research·En-Hua XiaXiao-Chun Wan
Oct 30, 2020·The Plant Journal : for Cell and Molecular Biology·Weiyi ZhangWeiwei Wen
Jan 1, 2021·Horticulture Research·En-Hua XiaXiao-Chun Wan
Apr 16, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Xi ZhuYunpeng Cao
Apr 3, 2018·Journal of Agricultural and Food Chemistry·Wenzhao WangTao Xia

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Datasets Mentioned

BETA
KY615688
KY615689

Methods Mentioned

BETA
transgenic
PCR
Assay

Software Mentioned

ESpript
ExPASy ProtParam tool
ClustalW
MEGA
PlantCARE

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