PMID: 9420227Jan 7, 1998Paper

Functional analysis of vaccinia virus B5R protein: essential role in virus envelopment is independent of a large portion of the extracellular domain

Journal of Virology
E HerreraS N Isaacs

Abstract

Vaccinia virus has two forms of infectious virions: the intracellular mature virus and the extracellular enveloped virus (EEV). EEV is critical for cell-to-cell and long-range spread of the virus. The B5R open reading frame (ORF) encodes a membrane protein that is essential for EEV formation. Deletion of the B5R ORF results in a dramatic reduction of EEV, and as a consequence, the virus produces small plaques in vitro and is highly attenuated in vivo. The extracellular portion of B5R is composed mainly of four domains that are similar to the short consensus repeats (SCRs) present in complement regulatory proteins. To determine the contribution of these putative SCR domains to EEV formation, we constructed recombinant vaccinia viruses that replaced the wild-type B5R gene with a mutated gene encoding a B5R protein lacking the SCRs. The resulting recombinant viruses produced large plaques, indicating efficient cell-to-cell spread in vitro, and gradient centrifugation of supernatants from infected cells confirmed that EEV was formed. In contrast, phalloidin staining of infected cells showed that the virus lacking the SCR domains was deficient in the induction of thick actin bundles. Thus, the highly conserved SCR domains present in...Continue Reading

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Citations

Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Minoru KidokoroHisatoshi Shida
Feb 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erika AssarssonAlessandro Sette
Mar 5, 2010·The Journal of General Virology·Adrien Breiman, Geoffrey L Smith
May 25, 2012·The Journal of General Virology·Virginie DoceulGeoffrey L Smith
Jan 30, 2015·The Journal of Antimicrobial Chemotherapy·Sophie DuraffourRobert Snoeck
Dec 15, 2015·Journal of Virology·Mercedes Fernández-EscobarSusana Guerra
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·W L ZeileF S Southwick
Oct 7, 2003·Annual Review of Microbiology·Geoffrey L SmithMansun Law
Aug 17, 2006·Journal of Virology·Beatriz Perdiguero, Rafael Blasco
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Kim Van VlietGrant McFadden
Jul 27, 2001·The Journal of Cell Biology·M HollinsheadG L Smith
Jan 25, 2003·Annual Review of Immunology·Bruce T SeetGrant McFadden
Apr 12, 2001·The Journal of General Virology·E C MathewG L Smith
Dec 6, 2002·The Journal of General Virology·Geoffrey L SmithMansun Law
Apr 26, 2007·Journal of Medical Virology·Majid LaassriKonstantin Chumakov
Apr 5, 2013·PLoS Pathogens·Jacquelyn HorsingtonTimothy P Newsome
May 6, 2011·Journal of Virology·Shannon McNultyDaniel Kalman
Jan 13, 2012·The Journal of General Virology·María M LorenzoRafael Blasco
Nov 8, 2017·Viruses·Graziele Pereira OliveiraJônatas Santos Abrahão
Aug 10, 1999·Journal of Virology·S B KapadiaH W Virgin
Sep 22, 2017·The Journal of General Virology·David C J CarpentierGeoffrey L Smith

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