Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB

The Journal of Microbiology
Seunghwa LeeJihwan Hwang

Abstract

Resistance-nodulation-division (RND) efflux pumps are associated with multidrug resistance in many gram-negative pathogens. The genome of Vibrio vulnificus encodes 11 putative RND pumps homologous to those of Vibrio cholerae and Escherichia coli. In this study, we analyzed three putative RND efflux pumps, showing homology to V. cholerae VexAB and VexCD and to E. coli AcrAB, for their functional roles in multidrug resistance of V. vulnificus. Deletion of the vexAB homolog resulted in increased susceptibility of V. vulnificus to bile acid, acriflavine, ethidium bromide, and erythromycin, whereas deletion of acrAB homologs rendered V. vulnificus more susceptible to acriflavine only. Deletion of vexCD had no effect on susceptibility of V. vulnificus to these chemicals. Upon exposure to these antibacterial chemicals, expression of tolCV1 and tolCV2, which are putative outer membrane factors of RND efflux pumps, was induced, whereas expression levels of vexAB, vexCD, and acrAB homologs were not significantly changed. Our results show that the V. vulnificus homologs of VexAB largely contributed to in vitro antimicrobial resistance with a broad substrate specificity that was partially redundant with the AcrAB pump homologs.

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Citations

Mar 28, 2018·International Journal of Molecular Sciences·Wen-Jung LuHong-Ting Victor Lin
Aug 19, 2015·Infection and Immunity·Saemee SongYong-Hak Kim
May 31, 2018·Microbial Genomics·Nicholas Petronella, Jennifer Ronholm
Jul 29, 2016·Clinical Microbiology Reviews·Jeticia R SistrunkChristina S Faherty
May 18, 2021·Frontiers in Cellular and Infection Microbiology·Yue GongYoung Ran Kim

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