Functional, anatomical, and molecular investigation of the cardiac conduction system and arrhythmogenic atrioventricular ring tissue in the rat heart

Journal of the American Heart Association
Andrew AtkinsonHalina Dobrzynski

Abstract

The cardiac conduction system consists of the sinus node, nodal extensions, atrioventricular (AV) node, penetrating bundle, bundle branches, and Purkinje fibers. Node-like AV ring tissue also exists at the AV junctions, and the right and left rings unite at the retroaortic node. The study aims were to (1) construct a 3-dimensional anatomical model of the AV rings and retroaortic node, (2) map electrical activation in the right ring and study its action potential characteristics, and (3) examine gene expression in the right ring and retroaortic node. Three-dimensional reconstruction (based on magnetic resonance imaging, histology, and immunohistochemistry) showed the extent and organization of the specialized tissues (eg, how the AV rings form the right and left nodal extensions into the AV node). Multiextracellular electrode array and microelectrode mapping of isolated right ring preparations revealed robust spontaneous activity with characteristic diastolic depolarization. Using laser microdissection gene expression measured at the mRNA level (using quantitative PCR) and protein level (using immunohistochemistry and Western blotting) showed that the right ring and retroaortic node, like the sinus node and AV node but, unlike v...Continue Reading

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Citations

Mar 3, 2015·Frontiers in Physiology·Thomas A CsepeVadim V Fedorov
Feb 28, 2018·Journal of Anatomy·Wiesława Klimek-PiotrowskaMateusz K Hołda
Aug 26, 2015·Circulation. Arrhythmia and Electrophysiology·Ning LiVadim V Fedorov
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Feb 13, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Minna HassinenMatti Vornanen
May 31, 2021·Journal of Cardiovascular Electrophysiology·Jon W Lomasney, Rachel Kaplan
Jul 2, 2021·Current Cardiology Reports·Sruthi MantriWilliam R Goodyer

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Methods Mentioned

BETA
PCR
light microscopy
protein assay
electrophoresis
PCRs

Software Mentioned

Pascal
WinEDR
Axiovision
MATLAB
WinWCP
Sigma Plot
StatMiner
Image Studio Lite
limma
Systat

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