PMID: 8964975Jan 1, 1996Paper

Functional and biochemical analysis of CNS-relevant neurotrophic activity in the lesioned sciatic nerve of adult rats

Journal für Hirnforschung
J Mey, S Thanos

Abstract

Prolonged posttraumatic survival and axonal elongation of adult retinal ganglion cells (RGC) depends drastically on the external supply of neurotrophic factors or on grafting of factor-producing peripheral nerve pieces to the site of lesion. As reported previously, an exudate collected in vivo from the regenerating sciatic nerve (ScN) of the rat, enhances axonal regeneration of the axotomized adult retinal ganglion cells (RGC) in vitro and retards their degeneration after optic nerve transection in vivo. In the present study, we analyzed the pool of secretable proteins during the posttraumatic phase of sciatic nerve injury and tested their neurotrophic potential on axotomized RGC. The proteins were accumulated within a silicon tube implanted for 1 week in situ. The sciatic nerve was either ligated to degenerate or crushed to regenerate or left intact. The conditioned media, collected according to the three protocols, were compared with regard to their neurotrophic activities both in vivo and in vitro. Proteins were labelled with [35S]-methionine and analyzed biochemically and autoradiographically. All samples had neuroprotective effects when injected into the vitreous body at the time of optic nerve transection and had neuritog...Continue Reading

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