Functional and chromosomal clustering of genes responsive to 5-bromodeoxyuridine in human cells

Experimental Gerontology
Sachi MinagawaDai Ayusawa

Abstract

5-Bromodeoxyuridine immediately and dramatically induces senescence-associated genes in human cells. We examined changes in gene expression in HeLa cells using cDNA microarrays containing ca. 39,000 human genes or ESTs. Addition of 5-bromodeoxyuridine for 4 days changed expression of 2.6% of them twice more (1.5%) or less (1.1%) than control levels. We functionally categorized 191 genes that showed greater than four times increase or decrease, and found that they have a various function. These genes were assigned to various human chromosomes, and half of them seemed to cluster at a few regions on individual chromosomes. These results suggest that multiple genes collectively act to induce cellular senescence and chromatin structure has a role in expression of the genes.

References

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Citations

Mar 31, 2010·Molecular Genetics and Genomics : MGG·Michihiko FujiiDai Ayusawa
Feb 19, 2010·Journal of the Association for Research in Otolaryngology : JARO·Jialin ShangJennifer Stone
Jun 26, 2007·Biogerontology·Palaniyappan Arivazhagan, Dai Ayusawa
Jan 22, 2009·Biogerontology·Vladimir N AnisimovGraham Pawelec
Nov 10, 2006·Biochemical and Biophysical Research Communications·Mohammad Nazir HossainDai Ayusawa
Aug 9, 2005·Biochemical and Biophysical Research Communications·Emi SumikawaDai Ayusawa
Mar 8, 2005·Experimental Cell Research·Sachi MinagawaDai Ayusawa
Feb 9, 2008·Biochemical and Biophysical Research Communications·Kensuke MikiDai Ayusawa
Jun 30, 2011·Biochemical and Biophysical Research Communications·Shinichi TakayamaDai Ayusawa
Nov 19, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J C de AlmeidaJ C Viana
Sep 7, 2006·Birth Defects Research. Part A, Clinical and Molecular Teratology·Sukhdeep Kaur Sahambi, Barbara F Hales
Mar 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryo UkekawaDai Ayusawa
Oct 16, 2015·Molecular and Cellular Biochemistry·Yoshimi YamakamiDai Ayusawa

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