Functional and composition differences between mitochondrial complex II in Arabidopsis and rice are correlated with the complex genetic history of the enzyme.

Plant Molecular Biology
Shaobai HuangA Harvey Millar

Abstract

Complex II plays a central role in mitochondrial metabolism as a component of both the electron transport chain and the tricarboxylic acid cycle. However, the composition and function of the plant enzyme has been elusive and differs from the well-characterised enzymes in mammals and bacteria. Herewith, we demonstrate that mitochondrial Complex II from Arabidopsis and rice differ significantly in several aspects: (1) Stability-Rice complex II in contrast to Arabidopsis is not stable when resolved by native electrophoresis and activity staining. (2) Composition-Arabidopsis complex II contains 8 subunits, only 7 of which have homologs in the rice genome. SDH 1 and 2 subunits display high levels of amino acid identity between two species, while the remainder of the subunits are not well conserved at a sequence level, indicating significant divergence. (3) Gene expression-the pairs of orthologous SDH1 and SDH2 subunits were universally expressed in both Arabidopsis and rice. The very divergent genes for SDH3 and SDH4 were co-expressed in both species, consistent with their functional co-ordination to form the membrane anchor. The plant-specific SDH5, 6 and 7 subunits with unknown functions appeared to be differentially expressed in ...Continue Reading

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Citations

Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Cynthia GleasonKaram B Singh
Aug 16, 2011·Plant Physiology·Jennifer KlodmannHans-Peter Braun
Sep 7, 2011·Biochimica Et Biophysica Acta·Juri DubininFrank Colditz
Apr 25, 2012·Plant Molecular Biology·Katrin PetersHans-Peter Braun
May 23, 2012·The Plant Journal : for Cell and Molecular Biology·Diwakar DahalKathleen J Newton
Mar 5, 2013·Current Opinion in Plant Biology·Shaobai Huang, A Harvey Millar
Nov 17, 2012·Plant Signaling & Behavior·Shaobai Huang, A Harvey Millar
Dec 13, 2016·The Plant Journal : for Cell and Molecular Biology·Jennifer SenklerHans-Peter Braun
Apr 8, 2018·The Biochemical Journal·Marcos Caballero-MoladaRamón Serrano
Jan 4, 2019·The Plant Journal : for Cell and Molecular Biology·Shaobai HuangA Harvey Millar
Feb 24, 2017·Frontiers in Plant Science·Jakob PetereitHolger Eubel
Mar 2, 2019·Annual Review of Plant Biology·Etienne H MeyerChris Carrie
Dec 31, 2020·International Journal of Molecular Sciences·Chun LiXiao-Jian Deng
Jul 15, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Irina V UkolovaVictor K Voinikov
Jun 12, 2013·Journal of Proteome Research·Clark J NelsonA Harvey Millar

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