Functional and molecular characterization of NHE3 expression during ontogeny in rat jejunal epithelium

The American Journal of Physiology
J F CollinsFayez K Ghishan

Abstract

Ontogenic changes occur in intestinal brush-border membrane vesicle (BBMV) Na+/H+ exchange activity. The present studies were designed to investigate ontogenic changes in Na+/H+ exchanger (NHE) isoform 3 in rat jejunum. pH-dependent Na+ uptake was assayed in four age groups of rats in the presence of 0, 50, or 800 microM HOE-694, a specific NHE inhibitor with differential sensitivities for NHE2 [inhibition constant (Ki) = 5 microM in PS120 fibroblasts] and NHE3 (Ki = 650 microM). Results showed that NHE2 and NHE3 contribute to basal BBMV uptake at all ages. Uptake levels were highest in 6-wk-old rats, lower in adult rats, and lowest in 2-wk-old (suckling) and 3-wk-old (weanling) rats, NHE3 contribution ranged from 92% at 6 wk of age to 59% at 2 and 3 wk of age. NHE3 inhibition by 800 microM HOE-694 was 38-45%. Statistical analysis showed that HOE-694 had a significant effect at both concentrations at all ages and that differences were present between all ages except 2- and 3-wk rats (at all HOE-694 concentrations). Northern blot analyses of jejunal mucosa showed lowest NHE3 mRNA levels in 2-wk animals and higher levels in all other age groups. Polyclonal antibodies were developed against an NHE3 COOH-terminal fusion protein, an...Continue Reading

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Citations

Sep 26, 2000·American Journal of Physiology. Cell Physiology·L BaiF K Ghishan
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jaleh MalakootiKrishnamurthy Ramaswamy
Feb 26, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua XuFayez K Ghishan
Dec 15, 2005·American Journal of Physiology. Renal Physiology·Shadi OweisJiang Liu
Sep 15, 2006·American Journal of Physiology. Cell Physiology·Dongsheng WangC Chris Yun
Jul 31, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua XuFayez K Ghishan
May 12, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua XuFayez K Ghishan
Dec 12, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua XuFayez K Ghishan
Mar 20, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hong C LiManoocher Soleimani
Jul 15, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hua XuFayez K Ghishan
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