Functional and molecular characterization of NOS isoforms in rat neutrophil precursor cells

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Sachin KumarMadhu Dikshit

Abstract

Previous studies from this laboratory have demonstrated importance of neutrophil-derived nitric oxide (NO) in free radical generation, characterized nitric oxide synthase (NOS) isoforms, and have reported subcellular distribution of NOS in rat peripheral neutrophils. Maximum number of neutrophils are added per day to the circulation from bone marrow, thus neutrophils might add substantial amount of NO in the bone marrow. NO generating ability and NOS isoforms characteristics in bone marrow neutrophil precursor cells is, however, still unexplored. This study was, therefore, undertaken to investigate NO generation ability and the molecular/biochemical characteristics of NOS isoforms in neutrophil precursor cells. The neutrophil precursors were separated on Percoll density gradient and characterized by Giemsa staining, CD markers, and by their size and granularity at various stages of maturation as Bands 1, 2, and 3. Mature neutrophils were efficient in free radical generation and phagocytosis, whereas immature cells had more mitochondria and myeloperoxidase. Amount of NO augmented from immature to mature neutrophils as assessed by fluorescent probe DAF-2DA and Griess reagent. Measurement of NOS enzyme activity further confirmed t...Continue Reading

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Citations

Oct 19, 2011·The Journal of Clinical Investigation·Ricky CheungRobert H Pierce
Aug 21, 2012·Human Immunology·Boris K PliyevMikhail Menshikov
Oct 5, 2018·Journal of Leukocyte Biology·Rashmi Saini, Sarika Singh
Mar 13, 2019·Journal of Leukocyte Biology·Samreen SadafMadhu Dikshit
Mar 28, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Samreen SadafMadhu Dikshit

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