Functional and Morphologic Alterations in Mechanical, Polymodal, and Cold Sensory Nerve Fibers of the Cornea Following Photorefractive Keratectomy

Investigative Ophthalmology & Visual Science
Federico BechCarlos Belmonte

Abstract

To define the characteristics and time course of the morphologic and functional changes experienced by corneal sensory nerves after photorefractive keratectomy (PRK). Unilateral corneal excimer laser photoablation was performed in 54 anesthetized 3- to 6-month-old mice; 11 naïve animals served as control. Mice were killed 0, 3, 7, 15, and 30 days after PRK. Excised eyes were placed in a recording chamber superfused at 34°C. Electrical nerve impulse activity of single sensory terminals was recorded with a micropipette applied onto the corneal surface. Spontaneous and stimulus-evoked (cold, heat, mechanical, and chemical stimuli) nerve terminal impulse (NTI) activity was analyzed. Corneas were fixed and stained with anti-β-Tubulin III antibody to measure nerve density and number of epithelial nerve penetration points of regenerating subbasal leashes. Nerve fibers and NTI activity were absent in the injured area between 0 and 7 days after PRK, when sparse regenerating nerve sprouts appear. On day 15, subbasal nerve density reached half the control value and abnormally responding cold-sensitive terminals were recorded inside the lesion. Thirty days after PRK, nerve density was almost restored, active cold thermoreceptors were abund...Continue Reading

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Citations

Dec 4, 2019·Investigative Ophthalmology & Visual Science·Carl MarfurtJoseph L Mankowski
Aug 25, 2020·Translational Vision Science & Technology·Eduardo AnituaFrancisco Muruzabal
Jun 26, 2020·International Journal of Molecular Sciences·Bastián RiveraMaría Pertusa
Nov 25, 2020·International Journal of Molecular Sciences·Darine FakihStéphane Mélik Parsadaniantz
Dec 29, 2020·Frontiers in Cellular Neuroscience·Adrian Guerrero-MorenoAnnabelle Réaux-Le Goazigo
Oct 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fanny JoubertAnnabelle Réaux-Le Goazigo
Aug 23, 2021·The Ocular Surface·Sonja MertschStefan Schrader

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