Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice

Scientific Reports
Chloé MichaudelBernhard Ryffel

Abstract

Environmental air pollutants including ozone cause severe lung injury and aggravate respiratory diseases such as asthma and COPD. Here we compared the effect of ozone on respiratory epithelium injury, inflammation, hyperreactivity and airway remodeling in mice upon acute (1ppm, 1 h) and chronic exposure (1.5ppm, 2 h, twice weekly for 6 weeks). Acute ozone exposure caused respiratory epithelial disruption with protein leak and neutrophil recruitment in the broncho-alveolar space, leading to lung inflammation and airway hyperresponsiveness (AHR) to methacholine. All these parameters were increased upon chronic ozone exposure, including collagen deposition. The structure of the airways as assessed by automatic numerical image analysis showed significant differences: While acute ozone exposure increased bronchial and lumen circularity but decreased epithelial thickness and area, chronic ozone exposure revealed epithelial injury with reduced height, distended bronchioles, enlarged alveolar space and increased collagen deposition, indicative of peribronchiolar fibrosis and emphysema as characterized by a significant increase in the density and diameter of airspaces with decreased airspace numbers. In conclusion, morphometric numerica...Continue Reading

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Citations

Apr 26, 2019·Inhalation Toxicology·Gregory J SmithSamir N P Kelada
Jun 12, 2020·Physiological Reports·Gurpreet Kaur AulakhJaswant Singh
Jul 30, 2019·Frontiers in Immunology·Sharon MumbyIan M Adcock
Jun 28, 2020·Scientific Reports·Julie K WatsonDonna K Finch
May 6, 2019·American Journal of Respiratory and Critical Care Medicine·Chris C LimGeorge D Thurston
Apr 23, 2020·International Journal of Molecular Sciences·Eistine Boateng, Susanne Krauss-Etschmann
Dec 1, 2020·Environmental Health Perspectives·James G WagnerRobert M Tighe
Dec 8, 2020·Frontiers in Medicine·Alessandro Venosa
Feb 23, 2021·PloS One·William P PedersonJulie G Ledford
Nov 5, 2021·Current Environmental Health Reports·Christina M Eckhardt, Haotian Wu

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Methods Mentioned

BETA
lavages
ELISA
FACS

Software Mentioned

GraphPad
GraphPad Prism
NDP
FlowJo

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