Functional and morphological features of skeletal muscle from mutant mice lacking both type 1 and type 3 ryanodine receptors

The Journal of Physiology
T IkemotoM Endo

Abstract

1. We generated mice with targeted disruptions in the genes for both ryanodine receptor type 1 (RyR-1) and type 3 (RyR-3) to study the functional roles of RyR subtypes in skeletal muscle. 2. In permeabilized myocytes lacking both the RyRs, the Ca(2+)-induced Ca2+ release (CICR) mechanism was completely lost, and caffeine failed to induce Ca2+ release. 3. Replacement of potassium methanesulphonate in an experimental intracellular solution with choline chloride resulted in Ca2+ release in the wild-type muscle but not in the mutant muscle lacking RyR-1. 4. The double-mutant mice exhibited more severe muscular degeneration than RyR-1-deficient mice with formation of large vacuoles and swollen mitochondria while structural coupling between T-tubules and the sarcoplasmic reticulum was retained. 5. These results demonstrate that CICR is mediated solely by RyR-1 and RyR-3 in skeletal muscle cells, and suggest that RyR-1 is involved in Cl(-)-induced Ca2+ release. The results also suggest the presence of molecular components other than RyRs responsible for the triad formation. RyR-3 may have a role in the normal morphogenesis of skeletal muscle cells, although functionally it can be replaced by RyR-1.

References

Jan 1, 1977·Physiological Reviews·M Endo
Feb 1, 1977·Circulation Research·A Fabiato, F Fabiato
Aug 1, 1989·The Journal of General Physiology·M Iino
Mar 1, 1980·Journal of Muscle Research and Cell Motility·M Endo, M Iino
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·H TakekuraC Franzini-Armstrong
Jan 28, 1993·Nature·M J Berridge
Aug 16, 1996·The Journal of Biological Chemistry·H TakeshimaT Noda
Jan 30, 1971·FEBS Letters·B BarlogieM Makinose
Jan 30, 1971·FEBS Letters·M Makinose, W Hasselbach

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Citations

Jul 13, 2000·Biochemical and Biophysical Research Communications·M NishiH Takeshima
May 27, 2003·Cell Calcium·Jianjie Ma, Zui Pan
Jan 25, 2003·Cell Calcium·Daniela Rossi, Vincenzo Sorrentino
Nov 23, 2000·Current Opinion in Genetics & Development·V SorrentinoD Rossi
Apr 29, 2008·Learning & Memory·Nicoletta GaleottiCarla Ghelardini
Aug 25, 2009·BMC Developmental Biology·Perrine CastetsPascale Guicheney
Jun 5, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hiroshi Takeshima
Jun 5, 2001·Japanese Journal of Pharmacology·K Kitamura, J Yamazaki
May 10, 2011·Annual Review of Biochemistry·Silvia Carrasco, Tobias Meyer
Mar 11, 2010·FEBS Letters·Alexander KushnirAndrew R Marks
Mar 21, 1998·The Journal of Cell Biology·F ProtasiP D Allen
Dec 30, 1999·The Journal of Cell Biology·M NishiH Takeshima
Oct 13, 2009·Biochimica Et Biophysica Acta·Sadayuki MatudaKyoko Nakano
Feb 19, 2015·American Journal of Respiratory Cell and Molecular Biology·Chantal DonovanRoss Vlahos
Jun 20, 1998·The Journal of Biological Chemistry·L H JeyakumarS Fleischer
May 31, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Chi-Lun ChangJen Liou
Apr 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Takekura, C Franzini-Armstrong
Aug 26, 1998·FEBS Letters·M ShimutaH Takeshima
Jul 23, 2008·Journal of Neurochemistry·Nicoletta GaleottiCarla Ghelardini
May 4, 2002·Nature Cell Biology·Zui PanJianjie Ma
Oct 1, 2009·Physiological Reviews·Makoto Endo
Jun 18, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S KomazakiH Takeshima
Apr 1, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M PodestàA Daga
Mar 18, 2003·Journal of Cell Science·Alessandro PisanielloMarina Bouché
Jul 27, 2021·Frontiers in Molecular Neuroscience·Christopher A Piggott, Yishi Jin

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