Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27.

Journal of Bacteriology
Trevor D LawleyD E Taylor

Abstract

The conjugative transfer region 1 (Tra1) of the IncHI1 plasmid R27 was subjected to DNA sequence analysis, mutagenesis, genetic complementation, and an H-pilus-specific phage assay. Analysis of the nucleotide sequence indicated that the Tra1 region contains genes coding for mating pair formation (Mpf) and DNA transfer replication (Dtr) and a coupling protein. Insertional disruptions of 9 of the 14 open reading frames (ORFs) in the Tra1 region resulted in a transfer-deficient phenotype. Conjugative transfer was restored for each transfer mutant by genetic complementation. An intergenic region between traH and trhR was cloned and mobilized by R27, indicating the presence of an origin of transfer (oriT). The five ORFs immediately downstream of the oriT region are involved in H-pilus production, as determined by an H-pilus-specific phage assay. Three of these ORFs encode proteins homologous to Mpf proteins from IncF plasmids. Upstream of the oriT region are four ORFs required for plasmid transfer but not H-pilus production. TraI contains sequence motifs that are characteristic of relaxases from the IncP lineage but share no overall homology to known relaxases. TraJ contains both an Arc repressor motif and a leucine zipper motif. A ...Continue Reading

References

Jul 5, 1978·Journal of Molecular Biology·A Kingsman, N Willetts
Jan 1, 1991·Methods in Enzymology·N KlecknerS Gottesman
Feb 1, 1980·Journal of General Microbiology·D E Taylor, J G Levine
Jan 1, 1995·Annual Review of Biochemistry·E Lanka, B M Wilkins
Jun 1, 1994·Microbiological Reviews·L S FrostR A Skurray
Apr 1, 1993·Journal of Bacteriology·D MaherD E Taylor
Apr 28, 1997·Molecular & General Genetics : MGG·E CabezónF de la Cruz
Nov 14, 1997·Molecular Microbiology·D R Byrd, S W Matson
Jan 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·A FicaF Cabello
Jun 15, 1999·Plasmid·M M RookerD E Taylor
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
Feb 27, 2001·Nucleic Acids Research·M D ErmolaevaS L Salzberg

❮ Previous
Next ❯

Citations

Jul 2, 2008·Applied Microbiology and Biotechnology·Masaki ShintaniHideaki Nojiri
May 2, 2013·Plasmid·Denis Arutyunov, Laura S Frost
Jul 12, 2003·FEMS Microbiology Letters·T D LawleyL S Frost
Oct 23, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cynthia Portal-CelhayMartin J Blaser
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·Kyungwon LeeYunsop Chong
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Chris SmillieFernando de la Cruz
Apr 28, 2009·FEMS Microbiology Reviews·María Pilar Garcillán-BarciaFernando de la Cruz
Nov 19, 2009·FEMS Microbiology Reviews·Fernando de la CruzEllen L Zechner
Jan 15, 2008·Plasmid·James E GuntonDiane E Taylor
May 21, 2016·Frontiers in Microbiology·Eleonora TassinariAntonio Juárez
Feb 3, 2018·Environmental Microbiology Reports·M HüttenerA Juárez
Jul 17, 2019·Environmental Microbiology Reports·Ainara LuqueCristina Madrid
Aug 6, 2020·Frontiers in Molecular Biosciences·Marta GibertCarlos Balsalobre
Jun 10, 2017·Molecular Genetics and Genomics : MGG·Delfina LarreaMatxalen Llosa
Nov 3, 2020·Frontiers in Microbiology·Elizabeth A McMillanJonathan G Frye
Feb 11, 2021·Molecular Microbiology·Steven J HancockMark A Schembri
Jul 10, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Christopher J Harmer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.