Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients

The Journal of Allergy and Clinical Immunology
M M van den HeuvelR H Beelen

Abstract

Several studies suggest a role for dendritic cell in the pathogenesis of allergic disease. The purpose of this study was to compare function and phenotype of monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control subjects. MoDCs were developed by incubating adherent monocytes for 5 days with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the allogeneic mixed leukocyte reaction and an autologous specific antigen presentation. The morphology of the MoDCs was characteristic for immature dendritic cells. MoDCs from allergic asthmatic patients showed phenotypic differences in the expression of HLA-DR, CD11b, and the high-affinity receptor for IgE. A clearly enhanced accessory potential of MoDCs from atopic asthmatic patients in the mixed leukocyte reaction was also shown. Moreover, house dust mite-specific T-cell proliferation was increased. This study suggests the involvement of dendritic cells in the pathogenesis of atopic asthma by an increased immunostimulatory capacity of MoDCs.

References

Dec 1, 1994·Current Opinion in Immunology·S Romagnani
Feb 1, 1995·Immunology Today·A BonomoE M Shevach
Jun 1, 1996·Immunology Today·J H PetersF Steinbach

❮ Previous
Next ❯

Citations

Mar 17, 2004·Biochimica Et Biophysica Acta·W S Fred Wong, Khai Pang Leong
Oct 11, 2003·Pharmacology & Therapeutics·John W Upham, Philip A Stumbles
Sep 15, 1999·Allergy·N NovakT Bieber
Dec 22, 1999·Allergy·W J Fokkens
Nov 14, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Luciano CastielloDavid F Stroncek
Dec 14, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C LensmarA Roquet
Mar 6, 2002·Clinical and Experimental Immunology·S J FokkemaU van der Velden
May 20, 2003·Respirology : Official Journal of the Asian Pacific Society of Respirology·John W Upham
Jun 8, 1999·Annual Review of Immunology·M Wills-Karp
Mar 23, 2006·Annual Review of Immunology·Leo Lefrançois, Lynn Puddington
May 21, 2004·American Journal of Respiratory and Critical Care Medicine·Julie A LongJohn W Upham
Oct 8, 1999·The Journal of Clinical Investigation·H C Oettgen, R S Geha
Dec 12, 2001·Allergy·K S BabuS T Holgate
Jan 29, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Chuang-Ming Wang, Jing-Jing Chuang
Nov 1, 2008·Journal of Periodontal Research·G L Torres de HeensB G Loos
Apr 20, 2002·Current Opinion in Allergy and Clinical Immunology·B N Lambrecht
Jun 6, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J W UphamP M O'Byrne
Dec 12, 2012·Experimental and Therapeutic Medicine·Chunying BaiLifeng Gao
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K ReichC Neumann
Sep 17, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Philip A StumblesPatrick G Holt
Apr 8, 1999·Clinical and Experimental Immunology·M M van den HeuvelG Kraal
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H KahlertH Fiebig
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Henning WeigtArmin Braun
Aug 30, 2017·Frontiers in Immunology·Heleen VromanMirjam Kool
Jan 12, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Luciano CastielloDavid F Stroncek
Nov 15, 2001·The Journal of Allergy and Clinical Immunology·J BousquetUNKNOWN World Health Organization
Nov 9, 2000·American Journal of Rhinology·W J FokkensA KleinJan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.