Functional and physical interaction between yeast Hsp90 and Hsp70

Proceedings of the National Academy of Sciences of the United States of America
Andrea N KravatsSue Wickner

Abstract

Heat shock protein 90 (Hsp90) is a highly conserved ATP-dependent molecular chaperone that is essential in eukaryotes. It is required for the activation and stabilization of more than 200 client proteins, including many kinases and steroid hormone receptors involved in cell-signaling pathways. Hsp90 chaperone activity requires collaboration with a subset of the many Hsp90 cochaperones, including the Hsp70 chaperone. In higher eukaryotes, the collaboration between Hsp90 and Hsp70 is indirect and involves Hop, a cochaperone that interacts with both Hsp90 and Hsp70. Here we show that yeast Hsp90 (Hsp82) and yeast Hsp70 (Ssa1), directly interact in vitro in the absence of the yeast Hop homolog (Sti1), and identify a region in the middle domain of yeast Hsp90 that is required for the interaction. In vivo results using Hsp90 substitution mutants showed that several residues in this region were important or essential for growth at high temperature. Moreover, mutants in this region were defective in interaction with Hsp70 in cell lysates. In vitro, the purified Hsp82 mutant proteins were defective in direct physical interaction with Ssa1 and in protein remodeling in collaboration with Ssa1 and cochaperones. This region of Hsp90 is also...Continue Reading

References

Jul 9, 1992·Nature·H WiechU Jakob
Jul 1, 1995·Molecular and Cellular Biology·D F Nathan, S Lindquist
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S P Bohen, K R Yamamoto
Jan 1, 1997·Molecular and Cellular Biology·H C ChangS Lindquist
Mar 7, 1998·The Journal of Biological Chemistry·B D JohnsonD O Toft
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·W C SuhC A Gross
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C S GässlerB Bukau
Jul 29, 2000·The Journal of Biological Chemistry·B D JohnsonD O Toft
Jun 21, 2002·Methods in Enzymology·Fred Sherman
Aug 6, 2002·The Journal of Biological Chemistry·M Patricia HernándezDavid O Toft
Aug 16, 2005·The Journal of Biological Chemistry·Youtao Song, Daniel C Masison
Jan 13, 2006·Journal of Molecular Biology·Harald WegeleJohannes Buchner
Apr 21, 2006·Nature·Maruf M U AliLaurence H Pearl
Jun 30, 2006·Trends in Endocrinology and Metabolism : TEM·Didier Picard
Oct 3, 2008·Molecular Biology of the Cell·Gary A FlomJill L Johnson
May 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eric B BertelsenErik R P Zuiderweg
Jun 26, 2009·The Journal of Biological Chemistry·Otmar HainzlKlaus Richter
Oct 20, 2009·Nature Structural & Molecular Biology·Shinji TsutsumiLen Neckers
May 19, 2010·Molecular and Cellular Biology·Michael Reidy, Daniel C Masison
Apr 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marika MiotSue Wickner
Apr 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Olivier GenestSue Wickner
Oct 20, 2011·Biochimica Et Biophysica Acta·Jill L Johnson
Nov 9, 2011·Journal of Molecular Biology·Timothy O StreetDavid A Agard
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Atta AhmadErik R P Zuiderweg
Nov 6, 2012·Molecular Cell·Roman KitykMatthias P Mayer
Mar 20, 2013·Trends in Biochemical Sciences·Alina RöhlJohannes Buchner
May 28, 2013·Nature Structural & Molecular Biology·Ruifeng QiQinglian Liu
Oct 21, 2014·PLoS Genetics·Michael ReidyDaniel C Masison
Dec 10, 2014·Sub-cellular Biochemistry·Swati Baindur-HudsonGregory L Blatch

❮ Previous
Next ❯

Citations

Nov 8, 2018·The Journal of Biological Chemistry·Olivier GenestShannon M Doyle
Aug 1, 2019·Molecular Biology and Evolution·Linh T Bui, Erik J Ragsdale
Aug 30, 2020·Journal of Biochemical and Molecular Toxicology·Derya KarabulutBirkan Yakan
Feb 13, 2019·Cold Spring Harbor Perspectives in Biology·Maximilian M Biebl, Johannes Buchner
Jan 9, 2019·Nature Structural & Molecular Biology·Laura J BlairMehdi Mollapour
Nov 18, 2019·Biophysics Reviews·Thiago V SeraphimWalid A Houry
Mar 25, 2020·Molecular and Cellular Biology·Chung-Hsuan KaoTanya T Paull
Jun 23, 2018·Genetics·Michael ReidyDaniel C Masison
Nov 27, 2020·Nature Communications·Kaushik BhattacharyaDidier Picard
Jun 21, 2020·Cytokine & Growth Factor Reviews·Anna Carolina Carvalho da FonsecaFlavia Regina Souza Lima
Aug 28, 2020·Cell Reports·Maximilian M BieblJohannes Buchner
Mar 7, 2021·International Journal of Molecular Sciences·Matthew P GrindleAndrea N Kravats
Mar 11, 2021·ACS Infectious Diseases·Tatyana Almeida TavellaFabio Trindade Maranhão-Costa
Apr 4, 2021·Journal of Fungi·Linda C Horianopoulos, James W Kronstad
Sep 4, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yu PanNa Song
Aug 11, 2021·Annual Review of Microbiology·Sue WicknerOlivier Genest
Oct 30, 2021·Frontiers in Molecular Biosciences·Li-Rong YanQian Xu
Dec 24, 2021·Nature·Ray Yu-Ruei WangDavid A Agard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.