Functional and selective RNA interference in developing axons and growth cones

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Ulrich HengstSamie R Jaffrey

Abstract

Developing axons and growth cones contain "local" mRNAs that are translated in response to various extracellular signaling molecules and have roles in several processes during axonal development, including axonal pathfinding, orientation of axons in chemotactic gradients, and in the regulation of neurotransmitter release. The molecular mechanisms that regulate mRNA translation within axons and growth cones are unknown. Here we show that proteins involved in RNA interference (RNAi), including argonaute-3 and argonaute-4, Dicer, and the fragile X mental retardation protein, are found in developing axons and growth cones. These proteins assemble into functional RNA-induced silencing complexes as transfection of small interfering RNAs selectively into distal axons results in distal axon-specific mRNA knock-down, without reducing transcript levels in proximal axons or associated diffusion of small interfering RNA into proximal axons or cell bodies. RhoA mRNA is localized to axons and growth cones, and intra-axonal translation of RhoA is required for growth cone collapse elicited by Semaphorin 3A (Sema3A), an axonal guidance cue. Selective knock-down of axonal RhoA mRNA abolishes Sema3A-dependent growth cone collapse. Our results dem...Continue Reading

Citations

Mar 20, 2010·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Martin MiklMichael A Kiebler
Dec 21, 2010·Histochemistry and Cell Biology·Isabell BastianRainer Viktor Haberberger
Jun 11, 2008·Current Neurology and Neuroscience Reports·Andreas Walter Kuss, Wei Chen
Feb 5, 2008·Nature Cell Biology·Andrew C Lin, Christine E Holt
Aug 4, 2009·Nature Cell Biology·Ian G MacaraStavroula Mili
Jan 15, 2008·Nature Cell Biology·Llewellyn J CoxSamie R Jaffrey
Jun 7, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Dónal O'Carroll, Anne Schaefer
Nov 23, 2006·Nature Reviews. Neuroscience·Kenneth S Kosik
Oct 18, 2007·Molecular BioSystems·Paul Lingor, Mathias Bähr
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Fumiko HoeftAllan L Reiss
Oct 2, 2013·The Journal of Cell Biology·Toshiaki ShigeokaChristine E Holt
Jul 10, 2012·Open Biology·Alessia Deglincerti, Samie R Jaffrey
May 15, 2007·Neuro-Signals·Raymond Chuen-Chung ChangCora Sau-Wan Lai
Mar 28, 2008·BMC Evolutionary Biology·Dennis MurphyJames R Brown
Nov 5, 2011·Molecular Brain·Liang HanJames Q Zheng
Aug 23, 2012·PloS One·Almudena Pacheco, Jeffery L Twiss
Oct 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Breset A WalkerSamie R Jaffrey
Jul 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yukio SasakiGary J Bassell
Nov 19, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Krishna H ZivrajChristine E Holt
Nov 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Armaz AschrafiBarry B Kaplan
Nov 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sandrine M GérantonStephen P Hunt
Apr 19, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yi ZhangZheng Gang Zhang
Mar 29, 2014·Nature Communications·Neilia G GraciasUlrich Hengst
Oct 21, 2009·Journal of Biomedicine & Biotechnology·Sabata MartinoAldo Orlacchio
Aug 13, 2011·Cellular and Molecular Life Sciences : CMLS·N F M Olde LoohuisA Aschrafi
Feb 20, 2009·Journal of Neuropathology and Experimental Neurology·Cory C TothDouglas W Zochodne
May 3, 2011·Current Opinion in Genetics & Development·Hosung JungChristine E Holt
Feb 11, 2010·Molecular and Cellular Neurosciences·Armaz AschrafiBarry B Kaplan
Nov 3, 2009·Progress in Neurobiology·Ian N M Day, Rod J Thompson
May 30, 2008·Current Opinion in Neurobiology·Andrew C Lin, Christine E Holt
Jan 16, 2008·Biochimica Et Biophysica Acta·Roberto FioreGerhard Schratt
Sep 25, 2007·Brain Research·Christian BarbatoCarlo Cogoni
Jun 1, 2007·Progress in Neurobiology·Fanny MannGeneviève Rougon
May 5, 2007·Seminars in Cell & Developmental Biology·Ulrich Hengst, Samie R Jaffrey
Jun 6, 2009·The European Journal of Neuroscience·Theodore J Price, Sandrine M Géranton
May 7, 2009·Developmental Medicine and Child Neurology·Brian W HaasAllan L Reiss
Jan 27, 2009·Glia·Jonathan D VerrierLucia Notterpek
May 22, 2007·Developmental Neurobiology·Wenlan WangJeffery L Twiss
Feb 8, 2007·The European Journal of Neuroscience·Maria EymanAntonio Giuditta
Jul 31, 2007·The EMBO Journal·Andrew C Lin, Christine E Holt
Jun 20, 2012·The European Journal of Neuroscience·Marco TolinoMichael A Kiebler
Nov 3, 2010·Traffic·Christopher J DonnellyJeffery L Twiss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved