Functional and sequence characterization of coagulation factor IX/factor X-binding protein from the venom of Echis carinatus leucogaster

Biochemistry
Y L Chen, I H Tsai

Abstract

A new coagulation factor IX/factor X-binding protein (IX/X-bp) from Echis carinatus leucogaster venom has been purified and designated ECLV IX/X-bp. ECLV IX/X-bp binds factor IX and X in a Ca(2+)-dependent manner and is devoid of thrombin-inhibitory and platelet-aggregating activities. The apparent dissociation constants (Kd) for binding of ECLV IX/X-bp to factor IX and factor X are 6.6 and 125 nM, respectively. Upon the addition of Mg2+, the required Ca2+ concentration for optimal binding of ECLV IX/X-bp to factor IX and factor X was prominently reduced. Mg2+ also increases the affinity of factor X for the venom protein. Direct binding of IX/X-bp to factor IX and X could also be detected by far-Western blotting, and results of the experiment ruled out the lectin-like mechanism of ECLV IX/X-bp. The complete amino acid sequence and the disulfide pattern of ECLV IX/X-bp was deduced by enzymatic hydrolysis and automated sequencing of the S-pyridylethylated protein. The venom protein is a heterodimer with one subunit of 131 amino acid residues and another of 125 residues. Both subunits are homologous to each other and to other snake venom proteins of the C-type lectin superfamily.

References

Jun 16, 1995·The Journal of Biological Chemistry·F SekiyaT Morita

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Citations

Jun 22, 2002·Toxicon : Official Journal of the International Society on Toxinology·Bon-Hun KooKwang-Hoe Chung
Jun 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Wen-Hui LeeYun Zhang
Dec 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·F S Markland
Sep 23, 2003·Biochimica Et Biophysica Acta·Sameh SarrayNaziha Marrakchi
Sep 29, 1998·The European Journal of Neuroscience·C I SeidenbecherM R Kreutz
Dec 4, 2004·Protein Science : a Publication of the Protein Society·Palasingam PaaventhanPrasanna R Kolatkar
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Xiaolong XuYongshu Xie
Feb 3, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Xiaolong XuJiehua Li
Jan 5, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Dengke ShenQingde Su
Dec 8, 2004·Toxicon : Official Journal of the International Society on Toxinology·Tomohisa OgawaMotonori Ohno
Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongYun Zhang
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Takashi Morita
May 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Qiumin LuKenneth J Clemetson
Jul 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Franziska T Arlinghaus, Johannes A Eble
May 19, 2006·Pathophysiology of Haemostasis and Thrombosis·Takashi Morita
Sep 29, 2019·Toxins·Tarek Mohamed Abd El-AzizJames D Stockand
Apr 29, 2020·International Journal of Molecular Sciences·Vance G Nielsen
Feb 28, 2002·The Journal of Biological Chemistry·Roberto GuerrantiEnrico Marinello
May 1, 2021·International Journal of Molecular Sciences·Vance G Nielsen

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