Functional and structural characterization of Spl proteases from Staphylococcus aureus

Journal of Molecular Biology
Grzegorz M PopowiczTad A Holak

Abstract

Staphylococcus aureus is the major cause of nosocomial infections world-wide, with increasing prevalence of community-acquired diseases. The recent dramatic increase in multi-antibiotic resistance, including resistance to the last-resort drug, vancomycin, together with the lack of an effective vaccine highlight the need for better understanding of S.aureus pathogenicity. Comparative analysis of available bacterial genomes allows for the identification of previously uncharacterized S.aureus genes with potential roles in pathogenicity. A good example is a cluster of six serine protease-like (spl) genes encompassed in one operon, which encode for putative proteases with similarity to staphylococcal glutamylendopeptidase (V8 protease). Here, we describe an efficient expression system for the production of recombinant SplB and SplC proteases in Escherichia coli, together with structural and functional characterization of the purified enzymes. A unique mechanism of cytoplasm protection against activity of misdirected SplB was uncovered. Apparently, the co-translated signal peptide maintains protease latency until it is cleaved by the signal peptidase during protein secretion. Furthermore, the crystal structure of the SplC protease re...Continue Reading

References

May 23, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G L Archer
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Feb 17, 1999·The British Journal of Dermatology·W C Noble
Feb 17, 2001·Infection and Immunity·S B ReedK W Bayles
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Oct 18, 2002·The Journal of Biological Chemistry·Clara Hink-SchauerDieter E Jenne
Nov 20, 2002·Biological Chemistry·Grzegorz Dubin
May 27, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rebecca R RobertsRobert A Weinstein
Jan 30, 2004·Acta Crystallographica. Section D, Biological Crystallography·Lata PrasadLouis T J Delbaere
Jul 28, 2004·Trends in Microbiology·Jodi A Lindsay, Matthew T G Holden
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·V S Lamzin, K S Wilson

❮ Previous
Next ❯

Citations

Mar 27, 2012·Journal of Bacteriology·Mark A SchallenbergerFloyd E Romesberg
Apr 26, 2008·PLoS Pathogens·Blaise R Boles, Alexander R Horswill
Sep 25, 2007·Current Opinion in Structural Biology·Istvan Botos, Alexander Wlodawer
Nov 21, 2007·Cellular & Molecular Biology Letters·Benedykt Władyka, Katarzyna Pustelny
Dec 9, 2008·Journal of Molecular Biology·Gautier RobinJennifer L Martin
Jun 19, 2016·The Journal of Allergy and Clinical Immunology·Sebastian StentzelBarbara M Bröker
Dec 7, 2013·Acta Crystallographica. Section D, Biological Crystallography·Afshan BegumChristian Betzel
Apr 10, 2014·The Journal of Biological Chemistry·Katarzyna PustelnyGrzegorz Dubin
Jan 30, 2009·The Biochemical Journal·Justyna Stec-NiemczykGrzegorz Dubin
May 23, 2017·Frontiers in Cellular and Infection Microbiology·Giampiero PietrocolaPietro Speziale
Nov 30, 2018·Medical Microbiology and Immunology·Vigyasa Singh, Ujjal Jyoti Phukan
Mar 7, 2021·International Journal of Molecular Sciences·Natalia StachGrzegorz Dubin
Mar 16, 2019·Microbiology Spectrum·Kayan Tam, Victor J Torres

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.