Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel

Scientific Reports
Tong-Yi DouLing Yang

Abstract

Cellulosome is a kind of multienzyme complex that displays high activity, selectivity, and stability. Here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the Cellulosimicrobium cellulans wild-type strain F16 isolated from soil microflora. This multienzyme complex, with excellent catalytic efficiency of kcat 13.2 s(-1) to remove the C-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-DAXP), has an outstanding tolerance against organic solvents and an excellent general stability, with the long half-life of 214 hours. This cellulosome-like multienzyme complex has a novel structure distinct from the well-documented ones. The key catalytic subunit responsible for the β-xylosidase activity against 10-DAXP is identified to be a novel protein, indicating a new glycoside hydrolase (GH) family. The pioneering work described here offers a novel nanoscale biocatalyst for the production of biofuels and chemicals from renewable plant-based natural resources.

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Citations

Apr 21, 2018·Journal of Basic Microbiology·Da-Cheng HaoPei-Gen Xiao
Dec 15, 2018·Polish Journal of Microbiology·DA Cheng HaoPei Gen Xiao

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Datasets Mentioned

BETA
ATNL00000000
PRJNA209578

Methods Mentioned

BETA
gel filtration
Dynamic light scattering
electrophoresis

Software Mentioned

Annotation
SOAPdenovo
Armone
PredictProtein
GraphPad Prism
CAZymes Analysis Toolkit ( CAT )
ProteinPilot TM

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