Functional and structural roles of conserved cysteine residues in the carboxyl-terminal domain of the follicle-stimulating hormone receptor in human embryonic kidney 293 cells

Biology of Reproduction
Aída UribeA Ulloa-Aguirre

Abstract

The carboxyl-terminal segment of G protein-coupled receptors has one or more conserved cysteine residues that are potential sites for palmitoylation. This posttranslational modification contributes to membrane association, internalization, and membrane targeting of proteins. In contrast to other members of the glycoprotein hormone receptor family (the LH and thyroid-stimulating hormone receptors), it is not known whether the follicle-stimulating hormone receptor (FSHR) is palmitoylated and what are the effects of abolishing its potential palmitoylation sites. In the present study, a functional analysis of the FSHR carboxyl-terminal segment cysteine residues was carried out. We constructed a series of mutant FSHRs by substituting cysteine residues with alanine, serine, or threonine individually and together at positions 629 and 655 (conserved cysteines) and 627 (nonconserved). The results showed that all three cysteine residues are palmitoylated but that only modification at Cys629 is functionally relevant. The lack of palmitoylation does not appear to greatly impair coupling to G(s) but, when absent at position 629, does significantly impair cell surface membrane expression of the partially palmitoylated receptor. All FSHR Cys ...Continue Reading

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Citations

Apr 6, 2011·The Journal of Biological Chemistry·David M ZuckermanCarolyn E Machamer
Jan 12, 2010·The Journal of Clinical Endocrinology and Metabolism·Tsilya GerasimovaMaria D Lalioti
Aug 19, 2009·General and Comparative Endocrinology·B Levavi-SivanJ J Lareyre
Sep 18, 2015·Frontiers in Endocrinology·Mohammed Akli AyoubEric Reiter
Jun 29, 2013·Molecular and Cellular Endocrinology·Alfredo Ulloa-AguirreP Michael Conn
Aug 4, 2015·Frontiers in Endocrinology·Antara A Banerjee, Smita D Mahale
Oct 19, 2016·Molecular Pharmacology·Alfredo Ulloa-Aguirre, Teresa Zariñán
Dec 14, 2018·The Journal of Biological Chemistry·Naoko AdachiNaoaki Saito
Dec 18, 2018·Frontiers in Endocrinology·Alfredo Ulloa-AguirreJames A Dias
Nov 20, 2018·Frontiers in Endocrinology·Niamh Sayers, Aylin C Hanyaloglu
Dec 1, 2020·The FEBS Journal·Antara A BanerjeeSmita D Mahale

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