Functional and transcriptomic analysis of extracellular vesicles identifies calprotectin as a new prognostic marker in peripheral arterial disease (PAD)

Journal of Extracellular Vesicles
Goren Saenz-PipaonCarmen Roncal

Abstract

Peripheral arterial disease (PAD) is associated with a high risk of cardiovascular events and death and is postulated to be a critical socioeconomic cost in the future. Extracellular vesicles (EVs) have emerged as potential candidates for new biomarker discovery related to their protein and nucleic acid cargo. In search of new prognostic and therapeutic targets in PAD, we determined the prothrombotic activity, the cellular origin and the transcriptomic profile of circulating EVs. This prospective study included control and PAD patients. Coagulation time (Procoag-PPL kit), EVs cellular origin and phosphatidylserine exposure were determined by flow cytometry in platelet-free plasma (n = 45 PAD). Transcriptomic profiles of medium/large EVs were generated using the MARS-Seq RNA-Seq protocol (n = 12/group). The serum concentration of the differentially expressed gene S100A9, in serum calprotectin (S100A8/A9), was validated by ELISA in control (n = 100) and PAD patients (n = 317). S100A9 was also determined in EVs and tissues of human atherosclerotic plaques (n = 3). Circulating EVs of PAD patients were mainly of platelet origin, predominantly Annexin V positive and were associated with the procoagulant activity of platelet-free plas...Continue Reading

References

Jan 2, 2001·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·F ZeigerM Koksch
Oct 12, 2005·The Journal of Biological Chemistry·Michelle M McCormickCarolyn L Geczy
Jun 13, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Mihaela G IonitaDominique P V de Kleijn
May 4, 2010·PloS One·David Montaner, Joaquín Dopazo
Aug 31, 2011·Methods in Molecular Biology·Daniele MericoGary D Bader
Mar 14, 2012·Journal of Clinical Bioinformatics·Rizwan MasudIftikhar J Kullo
Aug 14, 2012·The Journal of Surgical Research·Roland S CronerWerner Lang
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Jul 27, 2014·International Journal of Cardiology·Georgios GiannopoulosSpyridon Deftereos
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
May 17, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Rolf P EngelbergerIris Baumgartner
Jan 19, 2016·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·B SigvantE Wahlberg
Feb 20, 2016·Journal of Cardiovascular Translational Research·Jeffrey R CrawfordMark L Entman
Mar 23, 2017·Journal of Extracellular Vesicles·Bogdan MateescuEsther N M Nolte-'t Hoen
May 14, 2017·Scientific Reports·Aizea Morales-KastresanaJennifer C Jones
Jul 2, 2017·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·T P SinghJ Golledge
May 5, 2018·Metabolism: Clinical and Experimental·Anouar Hafiane, Stella S Daskalopoulou
Oct 20, 2018·Nucleic Acids Research·Teng LiuAn-Yuan Guo
Nov 30, 2018·The New England Journal of Medicine·Ravi ShahJane E Freedman
Dec 1, 2018·The New England Journal of Medicine·Javier Riancho, Pascual Sánchez-Juan
Dec 14, 2018·International Journal of Molecular Sciences·Igor GiarrettaRoberto Pola

❮ Previous
Next ❯

Citations

Sep 10, 2020·International Journal of General Medicine·Shosaku NomuraTomoki Ito
Nov 3, 2020·Computational and Structural Biotechnology Journal·Yuchen LiShenglin Huang
Dec 20, 2020·International Journal of Molecular Sciences·Melania DovizioPaola Patrignani
Apr 4, 2021·International Journal of Molecular Sciences·Goren Saenz-PipaonCarmen Roncal
Jun 3, 2021·Journal of Clinical Medicine·Goren Saenz-PipaonCarmen Roncal

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE140320

Methods Mentioned

BETA
biopsy
amputation
flow cytometry
in vitro transcription
RNA-Seq
PCR
electrophoresis
ELISA
ELISAs
scRNA-Seq

Software Mentioned

tools
LimmaVoom
NOISeq R /
CytExpert
Bioconductor
STAR
mdGSA R package
Jeol
MARS
SPSS

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

Related Papers

International Journal of Molecular Sciences
Tünde SzatmáriKatalin Lumniczky
Cellular and Molecular Life Sciences : CMLS
Dimitry A ChistiakovYuri V Bobryshev
Journal of Visualized Experiments : JoVE
Kaori IshiguroTushar Patel
© 2021 Meta ULC. All rights reserved