Functional annotation and analysis of the Ornithodoros moubata midgut genes differentially expressed after blood feeding

Ticks and Tick-borne Diseases
Ana OleagaRicardo Pérez-Sánchez

Abstract

The argasid tick Ornithodoros moubata is the main vector of the African swine fever and the human relapsing fever in Africa. As part of the host-parasite-pathogen interface, the tick midgut expresses key proteins for tick survival and tick-borne pathogen transmission. Accordingly, midgut proteins are potential targets for the development of new drugs and vaccines aimed at tick control, and obtaining proteomic and transcriptomic data from the O. moubata midgut would facilitate the identification of such target candidates. With this aim, we have assembled and characterized the midgut transcriptome of O. moubata females before and 48h after a blood meal, and identified the genes that are differentially expressed in the midgut after feeding. Overall, 23,863 transcripts were obtained, and of them, 9,164 were identified and annotated. The most represented molecular functions were catalytic and binding activities, and the most represented biological processes were metabolic, cellular and single-organism processes. KEGG analysis of the annotated sequences assigned up to 3,053 of them to 130 active pathways, among which, the top 30 pathways were mostly metabolic routes. Differential expression analysis between unfed and fed ticks detect...Continue Reading

Citations

May 3, 2019·Scientific Reports·Ricardo N AraujoFabiano Oliveira
Aug 17, 2019·Acta Parasitologica·DongLing NiuLi Hu
Jun 2, 2018·Experimental & Applied Acarology·DongLing NiuLi Hu
Feb 6, 2021·PLoS Neglected Tropical Diseases·Ana OleagaRicardo Pérez-Sánchez
Feb 6, 2021·Journal of Insect Physiology·Claudio R LazzariMarcos H Pereira
Feb 26, 2021·Journal of Thermal Biology·Joanna M ReinholdChloé Lahondère

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