Functional annotation and kinetic characterization of PhnO from Salmonella enterica

Biochemistry
James C Errey, John S Blanchard

Abstract

Phosphorus is an essential nutrient for all living organisms. Under conditions of inorganic phosphate starvation, genes from the Pho regulon are induced, allowing microorganisms to use phosphonates as a source of phosphorus. The phnO gene was previously annotated as a transcriptional regulator of unknown function due to sequence homology with members of the GCN5-related N-acyltransferase family (GNAT). PhnO can now be functionally annotated as an aminoalkylphosphonic acid N-acetyltransferase which is able to acetylate a range of aminoalkylphosphonic acids. Studies revealed that PhnO proceeds via an ordered, sequential kinetic mechanism with AcCoA binding first followed by aminoalkylphosphonate. Attack by the amine on the thioester of AcCoA generates the tetrahedral intermediate that collapses to generate the products. The enzyme also requires a divalent metal ion for activity, which is the first example of this requirement for a GNAT family member.

References

May 22, 1970·Science·K E Kennedy, G A Thompson
Apr 19, 1966·Biochemical and Biophysical Research Communications·J F ChaseP K Tubbs
Jul 11, 2002·Journal of Bacteriology·Alexander D KimBrian M Martin
Dec 8, 2004·Archives of Biochemistry and Biophysics·Matthew W VettingJohn S Blanchard

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Citations

Nov 18, 2011·Nature·Siddhesh S KamatFrank M Raushel
Jun 28, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bjarne JochimsenBjarne Hove-Jensen
Apr 16, 2009·The Journal of Biological Chemistry·Kateryna PodzelinskaZongchao Jia
Jun 10, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kateryna PodzelinskaZongchao Jia
Nov 13, 2007·Journal of Bacteriology·Melanie A AdamsZongchao Jia
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ryo NasunoHiroshi Takagi
Jan 29, 2016·Biochemistry·Lorenza FavrotOlivia Vergnolle
Nov 2, 2015·Applied Microbiology and Biotechnology·Tatyana V ShushkovaAlexey A Leontievsky
Jul 23, 2013·Current Opinion in Chemical Biology·Spencer C Peck, Wilfred A van der Donk
Jul 9, 2013·Current Opinion in Chemical Biology·Siddhesh S Kamat, Frank M Raushel
Feb 12, 2010·FEMS Microbiology Letters·Terry J EvansGeorge P C Salmond
Oct 28, 2016·Chemical Reviews·Geoff P Horsman, David L Zechel
Sep 11, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Cemre ManavDitlev E Brodersen
Jul 17, 2015·Microbiology and Molecular Biology Reviews : MMBR·Kristy L Hentchel, Jorge C Escalante-Semerena
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Bjarne Hove-JensenBjarne Jochimsen
Apr 1, 2017·Frontiers in Cellular and Infection Microbiology·Johanna R ElfenbeinHelene L Andrews-Polymenis
Dec 4, 2019·Applied Biochemistry and Biotechnology·Natalia StosiekMagdalena Klimek-Ochab

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