Functional annotation of a novel toxin-antitoxin system Xn-RelT of Xenorhabdus nematophila; a combined in silico and in vitro approach

Journal of Molecular Modeling
Lalit Kumar GautamJitendra Singh Rathore

Abstract

Toxin-antitoxin (TA) complexes play an important role in stress responses and programmed cell death in bacteria. The RelB-RelE toxin antitoxin system is well studied in Escherichia coli. In this study, we used combined in silico and in vitro approaches to study a novel Xn-RelT toxin from Xenorhabdus nematophila bearing its own antitoxin Xn-RelAT-a RelB homolog of E. coli. The structure for this toxin-antitoxin pair is yet unknown. We generated homology-based models of X. nematophila RelT toxin and antitoxin. The deduced models were further characterized for protein-nucleic acid, protein-protein interactions and gene ontology. A detrimental effect of recombinant Xn-RelT on host E. coli was determined through endogenous toxicity assay. When expressed from a isopropyl β-D-1-thiogalactopyranoside-regulated LacZ promoter, Xn-RelT toxin showed a toxic effect on E. coli cells. These observations imply that the conditional cooperativity governing the Xn-RelT TA operon in X. nematophila plays an important role in stress management and programmed cell death.

References

Dec 5, 1997·Microbiology·Martin Holčík, V M Iyer
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A B de la HozP Ceglowski
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·S K ChristensenK Gerdes
Jan 31, 2003·Proteins·Simon C LovellDavid C Richardson
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Feb 19, 2005·Nucleic Acids Research·Deo Prakash Pandey, Kenn Gerdes
May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen
Sep 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eric R SchreiterCatherine L Drennan
Jul 10, 2007·Journal of Bacteriology·Roy David Magnuson
Jul 10, 2007·Nature Reviews. Microbiology·Erin E Herbert, Heidi Goodrich-Blair
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Nov 26, 2008·Journal of Bacteriology·Simon E S Bailey, Finbarr Hayes
Jan 24, 2009·Methods in Enzymology·Mikkel Christensen-DalsgaardKenn Gerdes
Dec 30, 2009·Journal of Bacteriology·Niles P DoneganAmbrose L Cheung
Jun 10, 2010·Annual Review of Microbiology·Kim Lewis
Nov 3, 2010·Current Opinion in Microbiology·Laurence Van Melderen
Nov 9, 2011·Annual Review of Genetics·Yoshihiro YamaguchiMasayori Inouye
Dec 30, 2014·TheScientificWorldJournal·Jitendra Singh Rathore, Lalit Kumar Gautam

❮ Previous
Next ❯

Citations

Feb 12, 2020·Applied Microbiology and Biotechnology·Mohit Yadav, Jitendra Singh Rathore

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis