PMID: 8967365Apr 1, 1996Paper

Functional antagonism between cAMP and cGMP on permeability of coronary endothelial monolayers

The American Journal of Physiology
A HempelH M Piper

Abstract

The role of the intracellular second messengers guanosine 3', 5'-cyclic monophosphate (cGMP) and adenosine 3', 5'-cyclic monophosphate (cAMP) in the control of macromolecule permeability was studied in cultured monolayers of microvascular coronary endothelial cells from rat. Macromolecule permeability was determined as passage of fluorescein isothiocyanate (FITC)-labeled albumin across the monolayers. Activation of adenylyl cyclase by the beta-adrenoceptor agonist isoproterenol (Iso; 10(-5) M) and the A2-adenosine receptor agonist 5'-(N-ethylcarboxamido)-adenosine (NECA; 10(-7) M) induced an increase in cellular cAMP contents that was accompanied by an increase in albumin flux. Effects of Iso and NECA on cellular cAMP level and albumin flux could be antagonized by a stimulator of the particular guanylyl cyclase, atrial natriuretic peptide (ANP; 10(-7) M), and stimulators of the soluble guanylyl cyclase, 3-morpholinosydnonimine (SIN-1; 10(-7) M) and sodium nitroprusside (SNP; 10(-6) M). ANP, SIN-1, and SNP also reduced cAMP content and basal macromolecule flux in unstimulated monolayers. 8-Bromoguanosine 3', 5'-cyclic monophosphate (8-BrcGMP; 5 x 10(-6) M), a stimulator of protein kinase G, reduced the increase in albumin flux u...Continue Reading

Citations

Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P HeF E Curry
Apr 17, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Virginia H HuxleyIngrid H Sarelius
Oct 12, 2004·American Journal of Physiology. Cell Physiology·K BindewaldT Noll
Feb 15, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Luis AgullóJordi Soler-Soler
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Luis AgullóJordi Soler-Soler
Nov 16, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tomoko IchikiJohn C Burnett
Jan 20, 2018·Contrast Media & Molecular Imaging·Anna VuorimaaLaura Airas
Nov 26, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Virginia H HuxleyStevan P Whitt
Mar 15, 2006·The Journal of Trauma·René RamirezGregory P Victorino

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